PPDB

The Plant Proteome Database

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AT5G66140.1   proteasome alpha subunit D2

Lab Annot. 20S proteasome alpha subunit D2 (PAD2)
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: proteasome core complex (sensu Eukaryota)9373170,9611183
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G51260.1 11Arabidopsis thaliana2E-1632299598233 (153)_
AT3G51260.2 11Arabidopsis thaliana2E-15521297100116 (70)_
AT3G22110.1 11Arabidopsis thaliana2E-532004266225 (155)M
Os09g36710.1 7Oryza sativa3E-153229899520 (20)_
Os08g43540.1 7Oryza sativa3E-153229899534 (34)S
Os06g07878.1 7Oryza sativa4E-55200436615 (15)M
v1_GRMZM2G138128_P02 4a53Zea mays6E-155250899569 (68)_
v1_GRMZM2G138128_P03 4a53Zea mays6E-155250899569 (68)_
GRMZM2G138128_P03 3.21Zea mays6E-155250899558 (58)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Proteasome(1) Proteasome_A_N(1)
TargetP: Cytoplasm (Class 3 C0.010; M0.400; S0.068; _0.813)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 250 aa (-cTP 206)
Molecular Weight 27.32 kDA(-cTP )
PI 8.73(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
135Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2193Detail        [A. thaliana] total soluble P3-strepII 26
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2253Detail       seedlings [A. thaliana] total tissue atg5
2297Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT

* For details about the exprimental sources click here.

Published Proteomics Data

15028209(Total chloroplast)
15215502(Vacuolar proteome)
18538804(total leaf)
19546170(mature pollen grains)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
20516081(tagged-26S proteasome-affinity)
21173025(root proteome)

Comparative Proteomics Data


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