PPDB

 The Plant Proteome Database

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AT5G65430.1   general regulatory factor 8
Lab Annot. 14-3-3 protein GF14 kappa (grf8)
Mapman: 30.7 signalling.14-3-3 proteins
Curated Location nucleus (Pubmed: 15659648 )

TAIR curated location: cell wall (sensu Magnoliophyta) & plasma membrane & nucleus & cytoplasm
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G65430.2 11Arabidopsis thaliana02489999329 (229)_
AT5G65430.3 11Arabidopsis thaliana1E-179242100100299 (196)_
AT5G10450.1 11Arabidopsis thaliana1E-1752489397495 (312)_
Os08g33370.1 7Oryza sativa1E-143239809168 (68)_
Os08g33370.2 7Oryza sativa1E-143239809168 (68)_
Os03g50290.2 7Oryza sativa5E-141238799277 (77)_
Zm00001eb179490_P001 agpv5Zea mays4E-14323979920 (0)_
Zm00001eb179490_P002 agpv5Zea mays4E-14323979920 (0)_
v1_GRMZM2G091155_P02 4a53Zea mays4E-1432397992158 (156)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: 14-3-3(1) PhoU_div(2)
TargetP: Cytoplasm (Class 3 C0.052; M0.182; S0.141; _0.637)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 248 aa (-cTP 213)
Molecular Weight 28.03 kDA(-cTP )
PI 4.82(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1508Detail        [A. thaliana]  
1515Detail       leaf [A. thaliana] wild-type
1516Detail       leaf [A. thaliana] wild-type
1517Detail       leaf [A. thaliana] wild-type
1518Detail       leaf [A. thaliana] wild-type
1519Detail       leaf [A. thaliana] wild-type
1520Detail       leaf [A. thaliana] wild-type
1521Detail       leaf [A. thaliana] wild-type
1522Detail       leaf [A. thaliana] wild-type
1523Detail       leaf [A. thaliana] wild-type
1524Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1615Detail       Natural Senescence leaf [A. thaliana]  
1616Detail       Natural Senescence leaf [A. thaliana]  
1617Detail        [A. thaliana] m48
1620Detail        [A. thaliana] m48
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1641Detail       leaf [A. thaliana] total tissue shoot1 mutant
1650Detail       leaf [A. thaliana] plastoglobules plastoglobulem48
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
1744Detail        [A. thaliana] Mep-6
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1984Detail       leaf [A. thaliana] plastid wild-type
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2003Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2004Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2218Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2220Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2254Detail       leaf [A. thaliana] total seedlings gfs9
2262Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2268Detail       seedlings [A. thaliana] total tissue wild-type
2274Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2398Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/35SRecA-GFP
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
14617066(Nuclear proteome)
16287169(Cell Wall proteome)
16619310(14-3-3 proteins)
18538804(total leaf)
17848588(total seedling)
19452453(14-3-3-interacting proteins)
18814325(cotyledons)
19200160(flower-stage 12)
20118269(developing seeds)
19525416(leaf (wt and clpr4-1 mutant))
19714877(interactomes 14-3-3 complexes (cell culture))
19888209(80S polysomal fraction)
19888209(leaves)
19423572(leaf (wt and clpr2-1))
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21173025(root proteome)

Comparative Proteomics Data

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