PPDB

PPDB	The Plant Proteome Database

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AT5G56730.1 Insulinase (Peptidase family M16) protein

Lab Annot.

peptidase M16 family (location not clear)
Mapman: 29.5.7.4.1* protein.degradation.metalloprotease.ME.M16

Curated Location

plastid (Pubmed: 23198870 )

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT5G42390.2 11 Arabidopsis thaliana 5E-25 614 22 40 0 (0) C
AT5G42390.1 11 Arabidopsis thaliana 5E-25 614 22 40 499 (296) C
AT1G06900.1 11 Arabidopsis thaliana 2E-06 182 25 46 20 (15) _
Os01g37825.1 7 Oryza sativa 0 999 70 83 7 (7) _
Os01g37825.2 7 Oryza sativa 0 826 69 83 4 (4) _
Os06g41990.2 7 Oryza sativa 1E-20 215 30 48 57 (57) C
GRMZM2G392649_P01 3.21 Zea mays 0 488 62 76 12 (12) _
Zm00001eb162240_P003 agpv5 Zea mays 0 990 71 84 0 (0) _
Zm00001eb162240_P001 agpv5 Zea mays 0 826 68 82 0 (0) _
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	Methyltransf_14(2) Peptidase_M16(2) Peptidase_M16_C(2)
TargetP:	Cytoplasm (Class 3 C0.030; M0.316; S0.085; _0.767)
Predotar:
Subcel. Location:
TM-HMM prediction:	1 TMs
Aramemnon:	2 TMs
TAT position:
Length	1008 aa (-cTP 984)
Molecular Weight	113.62 kDA(-cTP )
PI	5.49(-cTP )

Experimental Evidence

Expand

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
342	Detail								leaf [A. thaliana]	chloroplast low density membrane	wild-type
877	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1350	Detail								leaf [A. thaliana]	total tissue (5 day HL)	abck2xabck3 (rep. 3)
1397	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 3)
1421	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 1)
1423	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 3)
1463	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 3)
1934	Detail								leaf [A. thaliana]	chloroplast	wild-type
1935	Detail								leaf [A. thaliana]	chloroplast	wild-type
1937	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1938	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1983	Detail								leaf [A. thaliana]	plastid	wild-type
1986	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1987	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1988	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2

* For details about the exprimental sources click here.

Published Proteomics Data

16618929(MITO/PLASTID-LOPIT-callus)
18431481(chloroplast)
20061580(chloroplast envelope (inner+outer))
21311031(mitochondria)
21515685(GreenCut2)

Comparative Proteomics Data