PPDB

 The Plant Proteome Database

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AT5G40950.1   ribosomal protein large subunit 27
Lab Annot. 50S ribosomal protein L27
Mapman: 29.2.1.1.1.2.27 protein.synthesis.ribosomal protein.prokaryotic.chloroplast.50S subunit.L27
Curated Location plastid ribosome (Pubmed: 23198870 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G16930.1 11Arabidopsis thaliana2E-228361710 (0)M
AT2G16930.2 11Arabidopsis thaliana2E-228361710 (0)M
AT2G16930.4 11Arabidopsis thaliana2E-228361710 (0)M
Os01g69950.1 7Oryza sativa6E-76202627459 (59)C
Os04g25540.1 7Oryza sativa4E-227062770 (0)M
Os08g31219.1 7Oryza sativa6E-227061770 (0)M
Zm00001eb364200_P003 agpv5Zea mays2E-7620061750 (0)C
GRMZM2G145496_P01 3.21Zea mays2E-762006175226 (224)C
v1_GRMZM2G145496_P01 4a53Zea mays2E-762006175183 (183)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Ribosomal_L27(1)
TargetP: Chloroplast (Class 4 C0.709; M0.032; S0.392; _0.020)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 198 aa (-cTP 179)
Molecular Weight 21.74 kDA(-cTP 19.81)
PI 9.96(-cTP 9.92)

Experimental Evidence

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View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
30Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
151Detail       leaf [A. thaliana] chloroplast stromawild-type
303Detail       leaf [A. thaliana] chloroplast stromawild-type
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
324Detail       leaf [A. thaliana] cell wall enriched wild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
327Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
343Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
420Detail       leaf [A. thaliana] chloroplast stromaclpR1-stop (EMS)
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
541Detail       leaf [A. thaliana] stroma (FPLC) >1 MDa stromawild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
617Detail       leaf [A. thaliana] chloroplast stromawild-type
618Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
640Detail       leaf [A. thaliana] plastid nucleoid wild-type
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
876Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1175Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1196Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1214Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1215Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1331Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1420Detail        [A. thaliana]  
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1427Detail        [A. thaliana] total leaf tissue 1.11 wild-type
1428Detail        [A. thaliana] total leaf tissue 1.11 Prep1_Prep2_t1_t2
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1454Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1475Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 4)
1477Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 5)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1507Detail        [A. thaliana] wild-type
1508Detail        [A. thaliana]  
1525Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1535Detail       leaf [A. thaliana] wild-type
1538Detail       leaf [A. thaliana] plastid RNCs thylakoid membranewild-type
1539Detail       leaf [A. thaliana] wild-type
1574Detail       leaf [A. thaliana] TiO2 thylakoid membranek2k3
1575Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1596Detail        [A. thaliana]  
1625Detail       Rosettes [A. thaliana] Wild-type
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1628Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopek1xk3
1635Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1636Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1638Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1641Detail       leaf [A. thaliana] total tissue shoot1 mutant
1643Detail       leaf [A. thaliana] total leaf tissue Wild-type
1670Detail        [A. thaliana]  
1672Detail        [A. thaliana]  
1676Detail       Rosettes [A. thaliana]  
1690Detail        [A. thaliana]  
1707Detail        [A. thaliana] sht1
1708Detail        [A. thaliana] Wild-type
1748Detail       leaf [A. thaliana] total leaf tissue Col_0
1749Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1756Detail       leaf [A. thaliana] total leaf tissue Col_0
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1758Detail       leaf [A. thaliana] total leaf tissue DXS_Flag+fosmidomycin
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1830Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Normal light
1870Detail       Total leaf [A. thaliana] Stroma wild-type
1873Detail       Total leaf [A. thaliana] Stroma wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1933Detail       leaf [A. thaliana] chloroplast wild-type
1934Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2021Detail       leaf [A. thaliana] total leaf tissue  
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2120Detail       leaf [A. thaliana] Stroma stromacGep
2121Detail       leaf [A. thaliana] Stroma stromacGep
2122Detail       leaf [A. thaliana] Stroma stromacGep
2123Detail       leaf [A. thaliana] Stroma stromaWild typre
2124Detail       leaf [A. thaliana] Stroma stromawild type
2125Detail       leaf [A. thaliana] Stroma stromawild type
2126Detail       leaf [A. thaliana] Stroma stromacGep
2127Detail       leaf [A. thaliana] Stroma stromacGep
2128Detail       leaf [A. thaliana] Stroma stromacGep
2129Detail       leaf [A. thaliana] Stroma stromawild-type
2130Detail       leaf [A. thaliana] Stroma stromawild-type
2131Detail       leaf [A. thaliana] Stroma stromawild-type
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2165Detail       leaf [A. thaliana] stroma Clp stromaP3-trap-strepII
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2190Detail        [A. thaliana] plastid stroma stromaP3-strepII 31
2191Detail        [A. thaliana] plastid stroma stromaP3-trap-strepII 40
2209Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 10
2210Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII st28
2211Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 12
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2217Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2236Detail       seedlings [A. thaliana] total tissue gfs9
2237Detail       seedlings [A. thaliana] total tissue gfs9
2238Detail       seedlings [A. thaliana] total tissue gfs9
2239Detail       seedlings [A. thaliana] total tissue gfs9
2240Detail       seedlings [A. thaliana] total tissue gfs9
2241Detail       seedlings [A. thaliana] total tissue gfs9
2242Detail       seedlings [A. thaliana] total tissue gfs9
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2254Detail       leaf [A. thaliana] total seedlings gfs9
2255Detail       leaf [A. thaliana] total seedlings gfs9
2256Detail       leaf [A. thaliana] total seedlings gfs9
2257Detail       leaf [A. thaliana] total seedlings gfs9
2259Detail       leaf [A. thaliana] total seedlings gfs9
2261Detail       leaf [A. thaliana] stroma Clp clp trap
2263Detail       leaf [A. thaliana] stroma Clp Clp C1 trap
2265Detail       leaf [A. thaliana] stroma Clp stromaClp C1 trap
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2267Detail       leaf [A. thaliana] stroma Clp Clp C1 TRAP
2284Detail        [A. thaliana] Clp C1 WT
2285Detail        [A. thaliana] Clp C1 TRAP
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2315Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
11826309(thylakoid peripheral&lumen)
16207701(chloroplast stroma)
18431481(chloroplast)
19200160(flower-stage 12)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19334764(plasma membrane (cell culture))
20423899(chloroplast)
19888209(80S polysomal fraction)
19423572(leaf (wt and clpr2-1))
20061580(chloroplast envelope (inner+outer))
19995723(chloroplast reference proteome)
21189294(nuclear pore complex (co-IPs RAE1,Nup43,50a,93a,136))

Comparative Proteomics Data

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