PPDB

 The Plant Proteome Database

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AT5G35220.1   Peptidase M50 family protein
Lab Annot. EGY1, metalloprotease
Mapman: 29.5.7.10.1* protein.degradation.metalloprotease.MM.M50
Curated Location thylakoid-integral (Pubmed: 23198870 15659096 18431481 )

TAIR curated location: chloroplast15659096
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G35210.1 11Arabidopsis thaliana8E-71124991001 (1)_
AT5G05740.2 11Arabidopsis thaliana3E-404912948154 (131)C
AT5G05740.3 11Arabidopsis thaliana3E-404912948137 (114)C
Os03g57840.1 7Oryza sativa0436768923 (23)C
Os03g57840.2 7Oryza sativa0436768923 (23)C
Os01g04900.1 7Oryza sativa3E-42539274655 (55)C
GRMZM2G007907_P01 3.21Zea mays0431778822 (22)C
v1_GRMZM2G007907_P01 4a53Zea mays0431778818 (18)C
Zm00001eb060350_P002 agpv5Zea mays045972840 (0)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Peptidase_M50(1)
TargetP: Chloroplast (Class 3 C0.688; M0.270; S0.027; _0.026)
Predotar:
Subcel. Location: Transmembrane
TM-HMM prediction: 8 TMs
Aramemnon: 8 TMs
TAT position:
Length 548 aa (-cTP 530)
Molecular Weight 59.50 kDA(-cTP 57.59)
PI 5.32(-cTP 5.19)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
343Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
876Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1596Detail        [A. thaliana]  
1625Detail       Rosettes [A. thaliana] Wild-type
1626Detail       Rosettes [A. thaliana] k1xk3
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1628Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopek1xk3
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1831Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Normal light
1833Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2133Detail       leaf [A. thaliana] chloroplast fractionation MSH1

* For details about the exprimental sources click here.

Published Proteomics Data

 18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
21515685(GreenCut2)

Comparative Proteomics Data

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