PPDB

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AT5G20980.1 methionine synthase 3

Lab Annot.

5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase (MS3)
Mapman: 13.1.3.4.3 amino acid metabolism.synthesis.aspartate family.methionine.methionine synthase

Curated Location

plastid stroma (Pubmed: 23198870 15024005 ) (supported by in-house exprements)

TAIR curated location: chloroplast15024005

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT5G20980.2 11 Arabidopsis thaliana 0 812 100 100 25 (13) C
AT5G17920.1 11 Arabidopsis thaliana 0 761 80 90 1921 (1303) _
AT5G17920.2 11 Arabidopsis thaliana 0 761 80 90 1793 (1175) _
Os12g42884.2 7 Oryza sativa 0 762 79 89 198 (198) _
Os12g42884.3 7 Oryza sativa 0 762 79 89 198 (198) _
Os12g42884.1 7 Oryza sativa 0 762 79 89 198 (198) _
v1_GRMZM2G453616_P01 4a53 Zea mays 0 764 78 88 80 (79) _
v1_GRMZM2G453616_P02 4a53 Zea mays 0 764 78 88 80 (79) _
v1_GRMZM2G165747_P02 4a53 Zea mays 0 764 78 88 80 (79) _
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	LCD1(1) Meth_synt_1(3) Meth_synt_2(2)
TargetP:	Chloroplast (Class 5 C0.844; M0.701; S0.004; _0.009)
Predotar:
Subcel. Location:	Lumen
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	812 aa (-cTP 779) (-lTP 712)
Molecular Weight	90.59 kDA(-cTP 87.14)(-lTP 79.48)
PI	8.17(-cTP 7.37)(-lTP 6.162)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
94	Detail								root [B. rapa]	plastid stroma
102	Detail								petal [B. oleracea]	plastid stroma	Alboglabra
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
339	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
869	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
874	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2117	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2184	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2286	Detail								[A. thaliana]		Clp C1 WT
2316	Detail								leaf [A. thaliana]	PG plastoglobule

* For details about the exprimental sources click here.

Published Proteomics Data

18463617(suspension cells-phosphorylated proteins)
18431481(chloroplast)
18814325(cotyledons)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19114538(guard cells Arabidopsis leaf)
21311031(lysine-trimethylated leaf)
21311031(mitochondria)

Comparative Proteomics Data