PPDB

 The Plant Proteome Database

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AT5G20250.4   Raffinose synthase family protein
Lab Annot. alkaline alpha galactosidase (only model.4 has cTP)
Mapman: 3.1.2.2 minor CHO metabolism.raffinose family.raffinose synthases.putative
Curated Location plastid stroma (Pubmed: 23198870 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links PeptideAtlas   POGS   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G20250.2 11Arabidopsis thaliana0749100100162 (115)_
AT5G20250.1 11Arabidopsis thaliana0749100100162 (115)_
AT5G20250.3 11Arabidopsis thaliana0749100100162 (115)_
Os06g07600.1 7Oryza sativa0785637616 (16)M
Os08g38710.1 7Oryza sativa074657720 (0)_
Os07g10840.1 7Oryza sativa076253700 (0)_
GRMZM2G127147_P01 3.21Zea mays0771657927 (24)M
v1_GRMZM2G127147_P01 4a53Zea mays0771657925 (25)M
Zm00001eb281720_P001 agpv5Zea mays077165792 (2)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Raffinose_syn(1)
TargetP: Chloroplast (Class 3 C0.884; M0.345; S0.001; _0.017)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 844 aa (-cTP 768)
Molecular Weight 94.22 kDA(-cTP 85.39)
PI 6.99(-cTP 5.98)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1469Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 3)
1471Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clps (rep. 1-2)
1475Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 4)
1477Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 5)
1479Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 6)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1527Detail       leaf [A. thaliana] co-IP GST-ClpS 10ug bait stromaclpc1 x clps (rep. 2)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1756Detail       leaf [A. thaliana] total leaf tissue Col_0
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1758Detail       leaf [A. thaliana] total leaf tissue DXS_Flag+fosmidomycin
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2080Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2081Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2084Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2085Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2112Detail       leaf [A. thaliana] Stroma stromawt
2179Detail       leaf [A. thaliana] stroma Clp GST ClpF x ClpS1
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2300Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 

* For details about the exprimental sources click here.

Published Proteomics Data

 19995723(chloroplast reference proteome)
22060019(phosphoproteome - N-starved seedlings)
21533090(plasmodesmata-crude)
21173025(root proteome)
21768351(Leaf-Phosphoproteome)

Comparative Proteomics Data

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