PPDB

 The Plant Proteome Database

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AT5G16290.1   VALINE-TOLERANT 1
Lab Annot. acetolactate synthase small subunit
Mapman: 13.1.4.1.1 amino acid metabolism.synthesis.branched chain group.common.acetolactate synthase
Curated Location plastid (Pubmed: 23198870 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G16290.2 11Arabidopsis thaliana047710010068 (40)C
AT2G31810.1 11Arabidopsis thaliana04247083258 (155)C
AT2G31810.3 11Arabidopsis thaliana04246679207 (126)C
Os02g39570.1 7Oryza sativa0413738528 (28)C
Os02g39570.4 7Oryza sativa0410738528 (28)C
Os11g14950.1 7Oryza sativa040972840 (0)C
v1_GRMZM2G007647_P01 4a53Zea mays0411728323 (23)C
GRMZM2G007647_P01 3.21Zea mays0411728332 (32)C
Zm00001eb112480_P005 agpv5Zea mays041071820 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: ACT(2) ACT_4(2) ACT_5(3) Acylphosphatase(4) ALS_ss_C(2) DUF150_C(3) DUF4423(2) H
TargetP: Chloroplast (Class 4 C0.574; M0.203; S0.010; _0.100)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 477 aa (-cTP 415)
Molecular Weight 52.33 kDA(-cTP 45.67)
PI 6.78(-cTP 6.00)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2043Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2044Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2046Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2048Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2050Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2056Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2057Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2059Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2060Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2061Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2062Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2064Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2065Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2068Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2069Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2071Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2113Detail       leaf [A. thaliana] Stroma stromawt
2300Detail        [A. thaliana] Clp C1 TRAP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 17407188(cotyledon)
19525416(leaf (wt and clpr4-1 mutant))
21311031(chloroplasts)
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21841088(mitochondria (2DE native gel))
21173025(root proteome)

Comparative Proteomics Data

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