PPDB

The Plant Proteome Database

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AT5G08610.1   P-loop containing nucleoside triphosphate hydrolases superfamily protein

Lab Annot. DEAD box RNA helicase (RH26) (PDE340; Pigment defective embryo)
Mapman: 27.5* RNA.DEAD/DEAH BOX helicase
Curated Location plastid nucleoid (Pubmed: 23198870 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G08620.1 11Arabidopsis thaliana050188930 (0)_
AT5G63630.2 11Arabidopsis thaliana063172820 (0)M
AT5G63630.1 11Arabidopsis thaliana050581901 (1)_
Os01g43120.1 7Oryza sativa0473718537 (37)C
Os01g43130.1 7Oryza sativa047372854 (4)_
Os06g33520.1 7Oryza sativa054663783 (3)M
GRMZM2G041732_P01 3.21Zea mays055866805 (5)M
Zm00001eb383060_P004 agpv5Zea mays055866800 (0)M
v1_GRMZM2G041732_P01 4a53Zea mays055866809 (9)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: DEAD(1) Helicase_C(2) ResIII(2)
TargetP: Chloroplast (Class 5 C0.413; M0.329; S0.014; _0.020)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 850 aa (-cTP 778)
Molecular Weight 94.19 kDA(-cTP 86.04)
PI 9.50(-cTP 9.14)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1175Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1196Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1454Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 2)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1525Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1670Detail        [A. thaliana]  
1672Detail        [A. thaliana]  
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2239Detail       seedlings [A. thaliana] total tissue gfs9

* For details about the exprimental sources click here.

Published Proteomics Data

16807317(Phosphoproteins)
18463617(suspension cells-phosphorylated proteins)
18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
19376835(leaf phosphoproteins)
22060019(phosphoproteome - N-starved seedlings)
20733066(phosphoproteome-ABA-leaf)
21768351(Leaf-Phosphoproteome)

Comparative Proteomics Data


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