PPDB

The Plant Proteome Database

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AT5G08540.1   ribosomal RNA small subunit methyltransferase J

Lab Annot. unknown protein - maybe RNA binding?
Mapman: 35.2 not assigned.unknown
Curated Location envelope-inner (Pubmed: 23198870 18431481 ) (supported by in-house exprements)

TAIR curated location: thylakoid membrane (sensu Viridiplantae) & chloroplast envelope15322131,12775770
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
Os04g44560.1 7Oryza sativa5E-122303617728 (28)C
Zm00001eb076700_P001 agpv5Zea mays1E-11925165830 (0)C
GRMZM2G039711_P01 3.21Zea mays1E-1192516583166 (164)C
v1_GRMZM2G039711_P01 4a53Zea mays1E-1192516583102 (102)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: DUF3420(1) NuDC(2)
TargetP: Chloroplast (Class 3 C0.815; M0.397; S0.004; _0.034)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon: 1 TMs
TAT position:
Length 346 aa (-cTP 298)
Molecular Weight 38.65 kDA(-cTP 33.27)
PI 5.68(-cTP 5.20)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
105Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
107Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
343Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
876Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1262Detail       leaf [A. thaliana] co-IP SHOOT1 (rep. 2) thylakoid membranewild-type
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1548Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1549Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1551Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1552Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1553Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1554Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1555Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1569Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1572Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1574Detail       leaf [A. thaliana] TiO2 thylakoid membranek2k3
1576Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1577Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1578Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1580Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1581Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1620Detail        [A. thaliana] m48
1621Detail        [A. thaliana] m48
1622Detail        [A. thaliana] m48
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1628Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopek1xk3
1675Detail       Rosettes [A. thaliana]  
1678Detail       Rosettes [A. thaliana]  
1695Detail       Rosettes [A. thaliana] Thylakoids thylakoid membraneWild-type
1712Detail       Rosettes [A. thaliana] Thylakoids thylakoid membraneWild-type
1716Detail       Rosettes [A. thaliana] Thylakoids thylakoid membranek5-1
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2250Detail       seedlings [A. thaliana] total tissue atg5
2269Detail       seedlings [A. thaliana] total tissue wild-type
2298Detail        [A. thaliana] Clp C1 WT
2300Detail        [A. thaliana] Clp C1 TRAP
2304Detail        [A. thaliana] Clp C1 WT
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

12938931(Total chloroplast envelope)
12766230(Total chloroplast envelope)
15322131(Thylakoid-stripped)
18431481(chloroplast)
19995728(proplastid (Brassica napus))
19200160(flower-stage 12)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19376835(leaf phosphoproteins)
19423572(leaf (wt and clpr2-1))
20061580(chloroplast envelope (inner+outer))
19995723(chloroplast reference proteome)
21311031(chloroplasts)
21311031(mitochondria)
20733066(phosphoproteome-ABA-leaf)
21173025(root proteome)
21768351(Leaf-Phosphoproteome)
21515685(GreenCut2)

Comparative Proteomics Data


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