PPDB

PPDB	The Plant Proteome Database

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AT5G03650.1 starch branching enzyme 2.2

Lab Annot.

starch branching enzyme class II-1 (SBEII-1; BE1)
Mapman: 2.1.2.3 major CHO metabolism.synthesis.starch.starch branching

Curated Location

plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT2G36390.1 11 Arabidopsis thaliana 0 799 77 85 331 (267) C
AT3G20440.2 11 Arabidopsis thaliana 6E-147 583 40 61 18 (7) C
AT3G20440.3 11 Arabidopsis thaliana 4E-143 551 41 62 15 (5) C
Os02g32660.1 7 Oryza sativa 0 754 76 85 25 (25) C
Os02g32660.2 7 Oryza sativa 0 542 76 85 4 (4) C
Os06g51084.1 7 Oryza sativa 0 696 57 72 0 (0) C
GRMZM2G032628_P01 3.21 Zea mays 0 689 81 89 3 (3) C
Zm00001eb084160_P002 agpv5 Zea mays 0 642 84 92 0 (0) C
v1_GRMZM2G088753_P01 4a53 Zea mays 0 721 55 70 2 (2) C
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	Alpha-amylase(2) Alpha-amylase_C(1) CBM_48(1) hDGE_amylase(1)
TargetP:	Chloroplast (Class 5 C0.511; M0.427; S0.009; _0.083)
Predotar:
Subcel. Location:
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	805 aa (-cTP 744)
Molecular Weight	92.59 kDA(-cTP 85.95)
PI	5.54(-cTP 5.28)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
99	Detail								petal [B. oleracea]	plastid stroma
102	Detail								petal [B. oleracea]	plastid stroma	Alboglabra
151	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
180_181	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
187_188	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
195_194	Detail								leaf [A. thaliana]	chloroplast stroma	ffc1-2
249_250	Detail								leaf [A. thaliana]	chloroplast stroma	ffc1-2
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
327	Detail								leaf [A. thaliana]	chloroplast thylakoid membrane	wild-type
339	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
342	Detail								leaf [A. thaliana]	chloroplast low density membrane	wild-type
352	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
361	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
451	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
527	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt1)
544	Detail								leaf [A. thaliana]	stroma (FPLC) <600 kDa (LMA)	wild-type
618	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
621	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
622	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
640	Detail								leaf [A. thaliana]	plastid nucleoid	wild-type
641	Detail								[A. thaliana]	plastid nucleoid (crude)	wild-type
869	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
873	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
874	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
875	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
877	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1345	Detail								leaf [A. thaliana]	total tissue (5 day HL)	wild-type (rep. 1)
1346	Detail								leaf [A. thaliana]	total tissue (5 day HL)	wild-type (rep. 2)
1347	Detail								leaf [A. thaliana]	total tissue (5 day HL)	wild-type (rep. 3)
1349	Detail								leaf [A. thaliana]	total tissue (5 day HL)	abck2xabck3 (rep. 2)
1350	Detail								leaf [A. thaliana]	total tissue (5 day HL)	abck2xabck3 (rep. 3)
1399	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1400	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	wild-type
1401	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1402	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	wild-type
1403	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1404	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	wild-type
1405	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	wild-type
1407	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1410	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.2	wild-type
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1418	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 3)
1421	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 1)
1426	Detail								leaf [A. thaliana]	total leaf tissue	prep1xprep2xt1xt2 (rep. 3)
1431	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	wild-type (rep. 2)
1432	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	wild-type (rep. 2)
1439	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 1)
1440	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 2)
1441	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 3)
1442	Detail								leaf [A. thaliana]	total tissue (0 day HL)	abck2xabck3 (rep. 1)
1443	Detail								leaf [A. thaliana]	total tissue (0 day HL)	abck2xabck3 (rep. 2)
1444	Detail								leaf [A. thaliana]	total tissue (0 day HL)	abck2xabck3 (rep. 3)
1445	Detail								leaf [A. thaliana]	total tissue (3 day HL)	wild-type (rep. 1)
1447	Detail								leaf [A. thaliana]	total tissue (3 day HL)	wild-type (rep. 3)
1455	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1456	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1457	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1458	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 1)
1459	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 2)
1460	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 3)
1461	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 1)
1462	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 2)
1463	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 3)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1482	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 1)
1483	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1484	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 2)
1485	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1486	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 3)
1518	Detail								leaf [A. thaliana]		wild-type
1519	Detail								leaf [A. thaliana]		wild-type
1525	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 1)
1528	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 1)
1529	Detail								leaf [A. thaliana]	total leaf tissue - 0 uM CAP	wild-type (rep. 1)
1530	Detail								leaf [A. thaliana]	total leaf tissue - 30 uM CAP	wild-type (rep. 1)
1531	Detail								leaf [A. thaliana]	total leaf tissue - 40 uM CAP	wild-type (rep. 1)
1533	Detail								leaf [A. thaliana]	total leaf tissue - 30 uM CAP	clps (rep. 1)
1534	Detail								leaf [A. thaliana]	total leaf tissue - 40 uM CAP	clps (rep. 1)
1541	Detail								leaf [A. thaliana]	Thylakoids plastoglobule	M-48
1542	Detail								leaf [A. thaliana]		wild-type
1566	Detail								leaf [A. thaliana]	TiO2 thylakoid membrane	k2k3
1568	Detail								leaf [A. thaliana]	TiO2 thylakoid membrane	WT
1651	Detail								leaf [A. thaliana]	plastoglobules plastoglobule	wild-type
1826	Detail								leaf tip mesophyll [Zea Mays]	chloroplast stroma	W22 (repl. 3)
1872	Detail								Total leaf [A. thaliana]	Stroma	wild-type
1930	Detail								leaf [A. thaliana]	total leaf tissue	Col_0 Wild-type
1933	Detail								leaf [A. thaliana]	chloroplast	wild-type
1934	Detail								leaf [A. thaliana]	chloroplast	wild-type
1935	Detail								leaf [A. thaliana]	chloroplast	wild-type
1936	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1937	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1938	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1941	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1946	Detail								leaf [A. thaliana]	stroma UVR stroma	wild-type
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1948	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1949	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1983	Detail								leaf [A. thaliana]	plastid	wild-type
1984	Detail								leaf [A. thaliana]	plastid	wild-type
1985	Detail								leaf [A. thaliana]	plastid	wild-type
1986	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1987	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1988	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1999	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2000	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2001	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2002	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2009	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2010	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2011	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2012	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2013	Detail								leaf [A. thaliana]	total leaf tissue	wt
2014	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2016	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2017	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2077	Detail								Total leaf [A. thaliana]	total soluble	wt & clpt1xclpt2
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2113	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2115	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2117	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2142	Detail								Total leaf [A. thaliana]	total soluble stroma	wt & cgep
2144	Detail								Total leaf [A. thaliana]	total soluble stroma	wt & cgep
2148	Detail								Total leaf [A. thaliana]	total soluble stroma	wt & cgep
2150	Detail								Total leaf [A. thaliana]	total soluble stroma	wt & cgep
2182	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2183	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2184	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2185	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2224	Detail								leaf [A. thaliana]	total leaf tissue	Clp P3 strep
2225	Detail								leaf [A. thaliana]	total leaf tissue	Clp P3 trap
2266	Detail								leaf [A. thaliana]	stroma Clp	Clp C1 wild-type
2287	Detail								[A. thaliana]		Clp C1 WT
2290	Detail								[A. thaliana]		Clp C1 TRAP
2296	Detail								[A. thaliana]		Clp C1 WT
2300	Detail								[A. thaliana]		Clp C1 TRAP
2303	Detail								[A. thaliana]		Clp C1 WT
2307	Detail								[A. thaliana]		Clp C1 TRAP
2311	Detail								leaf [A. thaliana]	PG plastoglobule
2312	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2313	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2314	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2315	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2316	Detail								leaf [A. thaliana]	PG plastoglobule
2317	Detail								leaf [A. thaliana]	PG plastoglobule
2320	Detail								[A. thaliana]	Thylakoids plastoglobule
2321	Detail								leaf [A. thaliana]
2324	Detail								leaf [A. thaliana]	THYLAKOIDS thylakoid membrane

* For details about the exprimental sources click here.

Published Proteomics Data

16207701(chloroplast stroma)
15052571(soluble leaf)
18633119(stroma)
18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
20423899(chloroplast)
20706207(contaminants cell cycle interactome (cell culture))
19995723(chloroplast reference proteome)

Comparative Proteomics Data