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The Plant Proteome Database

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AT4G39080.1   vacuolar proton ATPase A3

Lab Annot. VHA-A3 (VACUOLAR PROTON ATPASE A3); ATPase
Mapman: 34.1 transport.p- and v-ATPases
Curated Location vacuole-tonoplast (Pubmed: 16461582 )

TAIR curated location: vacuolar membrane (sensu Magnoliophyta)16461582
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G21410.1 11Arabidopsis thaliana0811899466 (65)_
AT2G28520.1 11Arabidopsis thaliana080663791 (1)_
Os10g10500.1 7Oryza sativa0819768735 (35)_
Os03g14690.1 7Oryza sativa080976870 (0)_
Os01g61780.1 7Oryza sativa081261790 (0)_
Zm00001eb368270_P001 agpv5Zea mays067162790 (0)_
GRMZM2G104418_P02 3.21Zea mays039580900 (0)S
GRMZM2G104418_P01 3.21Zea mays039580900 (0)S
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: DUF4200(2) V_ATPase_I(1)
TargetP: Cytoplasm (Class 3 C0.323; M0.129; S0.085; _0.807)
Predotar:
Subcel. Location:
TM-HMM prediction: 6 TMs
Aramemnon: 6 TMs
TAT position:
Length 821 aa (-cTP 740)
Molecular Weight 92.83 kDA(-cTP )
PI 5.66(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
337Detail       bundle sheaths [A. thaliana]Bundle sheath total BS strands wild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1494Detail       leaf [A. thaliana]  
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1545Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1576Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1577Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1578Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1596Detail        [A. thaliana]  
1597Detail        [A. thaliana]  
1611Detail       leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1612Detail       Natural Senescence leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1613Detail       Natural Senescence leaf [A. thaliana]  
1614Detail       Natural Senescence leaf [A. thaliana]  
1615Detail       Natural Senescence leaf [A. thaliana]  
1616Detail       Natural Senescence leaf [A. thaliana]  
1617Detail        [A. thaliana] m48
1618Detail        [A. thaliana] m48
1619Detail        [A. thaliana] m48
1620Detail        [A. thaliana] m48
1621Detail        [A. thaliana] m48
1622Detail        [A. thaliana] m48
1625Detail       Rosettes [A. thaliana] Wild-type
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1628Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopek1xk3
1650Detail       leaf [A. thaliana] plastoglobules plastoglobulem48
1744Detail        [A. thaliana] Mep-6
1745Detail        [A. thaliana] Col-0
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1831Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Normal light
1832Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Normal light
1833Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2268Detail       seedlings [A. thaliana] total tissue wild-type
2269Detail       seedlings [A. thaliana] total tissue wild-type
2270Detail       seedlings [A. thaliana] total tissue wild-type
2308Detail       leaf [A. thaliana] PG plastoglobule 
2311Detail       leaf [A. thaliana] PG plastoglobule 
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

15215502(Vacuolar proteome)
15539469(vacuole)
16618929(vacuole-LOPIT-callus)
17293592(total seedlings û light and dark grown)
17151019(vacuole - suspension cell)
17644812(plasma membrane - suspension cells)
17432890(leaf total membranes)
17660356(tonoplast (Brassica oleracea buds))
18431481(chloroplast)
19546170(mature pollen grains)
19036721(detergent resistant plasma membrane (DRM) proteins (Cell cultures))
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19106119(plasma membrane detergent resistent microdomains (DRM))
19334764(plasma membrane (cell culture))
19376835(leaf phosphoproteins)
19114538(guard cells Arabidopsis leaf)
20061580(chloroplast envelope (inner+outer))
21826108(SYP trans-Golgi-filtered)
21533090(plasmodesmata-crude)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
20843791(DRM-enriched plasmamembrane)
21173025(root proteome)
22430844(Golgi-cell-culture)
21768351(Leaf-Phosphoproteome)
21988472(vacuole (unfiltered data))
21433285(Crude microsomes cell_cultures)
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data


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