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The Plant Proteome Database

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AT4G34350.1   4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Lab Annot. 1-hydroxy-2-methyl-butenyl 4-diphosphate reductase (HDR; previously ISPH, CLB6, lytB)
Mapman: 16.1.1.7 secondary metabolism.isoprenoids.non-mevalonate pathway.HDR
Curated Location plastid stroma (Pubmed: 23198870 15659625 18236010 18431481 )

TAIR curated location: chloroplast stroma15863698
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
Os03g52170.1 7Oryza sativa0444779017 (17)C
Os03g52180.1 7Oryza sativa2E-12424172850 (0)_
Os03g52210.1 7Oryza sativa1E-5216653690 (0)C
Zm00001eb056240_P002 agpv5Zea mays043177900 (0)C
GRMZM2G027059_P01 3.21Zea mays0431779020 (20)C
v1_GRMZM2G027059_P01 4a53Zea mays0431779013 (13)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: LYTB(1)
TargetP: Chloroplast (Class 5 C0.621; M0.536; S0.023; _0.043)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 466 aa (-cTP 428)
Molecular Weight 52.78 kDA(-cTP 48.48)
PI 5.76(-cTP 5.24)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
99Detail       petal [B. oleracea] plastid stroma 
195_194Detail       leaf [A. thaliana] chloroplast stromaffc1-2
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
641Detail        [A. thaliana] plastid nucleoid (crude) wild-type
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1456Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1469Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 3)
1479Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 6)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1637Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1985Detail       leaf [A. thaliana] plastid wild-type
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2141Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2142Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2143Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2144Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2145Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2147Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2149Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2150Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2151Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2152Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2290Detail        [A. thaliana] Clp C1 TRAP
2299Detail        [A. thaliana] Clp C1 TRAP
2310Detail       leaf [A. thaliana] PG plastoglobule 
2311Detail       leaf [A. thaliana] PG plastoglobule 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2319Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

18633119(stroma)
18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
20706207(contaminants cell cycle interactome (cell culture))
19995723(chloroplast reference proteome)
21166475(cytosolic-fractions-crude)
21173025(root proteome)
21472856(mitochondrial-2DEgels-uncurated)

Comparative Proteomics Data


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