PPDB

 The Plant Proteome Database

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AT4G19710.1   aspartate kinase-homoserine dehydrogenase ii
Lab Annot. aspartate kinase/homoserine dehydrogenase (ak-hsdh) model.2 has higher score than model.1
Mapman: 13.1.3.6.1.10 amino acid metabolism.synthesis.aspartate family.misc.homoserine.bifunctional aspartate kinase/homoserine dehydrogenase
Curated Location plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT4G19710.2 11Arabidopsis thaliana0836100100222 (164)C
AT1G31230.1 11Arabidopsis thaliana07698091471 (338)C
AT3G02020.1 11Arabidopsis thaliana2E-4549727510 (0)C
Os08g25390.1 7Oryza sativa0767758751 (51)C
Os09g12290.1 7Oryza sativa0754758711 (11)C
Os08g25390.2 7Oryza sativa0698778821 (21)C
GRMZM2G136712_P01 3.21Zea mays0503748528 (23)C
v1_GRMZM2G104546_P01 4a53Zea mays049473865 (5)_
v1_GRMZM2G136712_P01 4a53Zea mays0476748517 (17)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: AA_kinase(1) ACT(2) ACT_6(2) ACT_7(2) Homoserine_dh(1) NAD_binding_3(1)
TargetP: Chloroplast (Class 2 C0.945; M0.044; S0.007; _0.177)
Predotar:
Subcel. Location: Lumen
TM-HMM prediction: No
Aramemnon:
TAT position: 35
Length 859 aa (-cTP 833) (-lTP 793)
Molecular Weight 94.43 kDA(-cTP 91.63)(-lTP 87.19)
PI 6.35(-cTP 6.17)(-lTP 5.671)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
303Detail       leaf [A. thaliana] chloroplast stromawild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
620Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
871Detail       leaf [A. thaliana] chloroplast stromawild-type
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1597Detail        [A. thaliana]  
1933Detail       leaf [A. thaliana] chloroplast wild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2114Detail       leaf [A. thaliana] Stroma stromawt
2251Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2320Detail        [A. thaliana] Thylakoids plastoglobule 

* For details about the exprimental sources click here.

Published Proteomics Data

 18431481(chloroplast)
17216043(leaf)
20423899(chloroplast)
19114538(guard cells Arabidopsis leaf)
19995723(chloroplast reference proteome)
21693672(wound-regulated leaf proteome (wt))
21173025(root proteome)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
195_194ffc1-2 cICAT- exp1 / wt cICAT- exp1cICAT3GEMWSVHKFGGTCVGNSQR + ICAT_l; Ox / GEMWSVHKFGGTCVGNSQR + ICAT_h; Ox30.49 leaf [A. thaliana] chloroplast stroma

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