PPDB

 The Plant Proteome Database

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AT4G09000.1   general regulatory factor 1
Lab Annot. 14-3-3 protein GF14 chi (grf1)
Mapman: 30.7 signalling.14-3-3 proteins
Curated Location not plastid (supported by in-house exprements)

TAIR curated location: nucleus
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT4G09000.2 11Arabidopsis thaliana03188383542 (299)_
AT1G35160.1 11Arabidopsis thaliana3E-1782659096443 (263)_
AT1G78300.1 11Arabidopsis thaliana4E-1752609095524 (300)_
Os04g38870.5 7Oryza sativa7E-154249839258 (58)_
Os04g38870.3 7Oryza sativa7E-154249839258 (58)_
Os04g38870.2 7Oryza sativa7E-154249839258 (58)_
Zm00001eb080570_P001 agpv5Zea mays5E-15325082920 (0)_
Zm00001eb080570_P002 agpv5Zea mays5E-15325082920 (0)_
GRMZM2G102499_P04 3.21Zea mays5E-1532508292228 (135)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: 14-3-3(1) LsmAD(2) TPR_19(3) Virulence_fact(2)
TargetP: Cytoplasm (Class 3 C0.064; M0.065; S0.300; _0.783)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 267 aa (-cTP 262)
Molecular Weight 29.93 kDA(-cTP )
PI 4.68(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
324Detail       leaf [A. thaliana] cell wall enriched wild-type
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
395Detail       leaf [A. thaliana] total leaf tissue ffc1-2
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
617Detail       leaf [A. thaliana] chloroplast stromawild-type
619Detail       leaf [A. thaliana] chloroplast stromawild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1114Detail        [A. thaliana] RARE1 co-IP rare-1
1125Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1126Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1127Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1508Detail        [A. thaliana]  
1516Detail       leaf [A. thaliana] wild-type
1517Detail       leaf [A. thaliana] wild-type
1518Detail       leaf [A. thaliana] wild-type
1519Detail       leaf [A. thaliana] wild-type
1520Detail       leaf [A. thaliana] wild-type
1521Detail       leaf [A. thaliana] wild-type
1523Detail       leaf [A. thaliana] wild-type
1524Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1540Detail       leaf [A. thaliana] Thylakoids plastoglobulewild-type
1541Detail       leaf [A. thaliana] Thylakoids plastoglobuleM-48
1544Detail       leaf [A. thaliana] TiO2 thylakoid membranek1xk3
1545Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1546Detail       leaf [A. thaliana] TiO2 thylakoid membranek1xk3
1547Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1552Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1554Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1557Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1566Detail       leaf [A. thaliana] TiO2 thylakoid membranek2k3
1567Detail       leaf [A. thaliana] TiO2 thylakoid membranek2k3
1568Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1569Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1572Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1576Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1577Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1578Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1579Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1580Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1581Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1582Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1583Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1596Detail        [A. thaliana]  
1620Detail        [A. thaliana] m48
1621Detail        [A. thaliana] m48
1622Detail        [A. thaliana] m48
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1641Detail       leaf [A. thaliana] total tissue shoot1 mutant
1650Detail       leaf [A. thaliana] plastoglobules plastoglobulem48
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
1707Detail        [A. thaliana] sht1
1744Detail        [A. thaliana] Mep-6
1745Detail        [A. thaliana] Col-0
1756Detail       leaf [A. thaliana] total leaf tissue Col_0
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1758Detail       leaf [A. thaliana] total leaf tissue DXS_Flag+fosmidomycin
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1831Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Normal light
1832Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Normal light
1833Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1928Detail       Rosettes [A. thaliana] Thylakoids wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1984Detail       leaf [A. thaliana] plastid wild-type
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2003Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2004Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2028Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2112Detail       leaf [A. thaliana] Stroma stromawt
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2193Detail        [A. thaliana] total soluble P3-strepII 26
2208Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII
2209Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 10
2210Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII st28
2211Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 12
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2217Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2218Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2220Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2222Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2223Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2261Detail       leaf [A. thaliana] stroma Clp clp trap
2262Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2263Detail       leaf [A. thaliana] stroma Clp Clp C1 trap
2264Detail       leaf [A. thaliana] stroma Clp stromawild-type
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2267Detail       leaf [A. thaliana] stroma Clp Clp C1 TRAP
2268Detail       seedlings [A. thaliana] total tissue wild-type
2272Detail       seedlings [A. thaliana] total tissue wild-type
2284Detail        [A. thaliana] Clp C1 WT
2285Detail        [A. thaliana] Clp C1 TRAP
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2308Detail       leaf [A. thaliana] PG plastoglobule 
2311Detail       leaf [A. thaliana] PG plastoglobule 
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2319Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2398Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/35SRecA-GFP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
14617066(Nuclear proteome)
15539469(vacuole)
16358359(cell suspension (Gamborg))
16502469(cell suspension-CD-down)
16619310(14-3-3 proteins)
15276459(epidermis)
17317660(Plasma Membrane proteome)
18538804(apoplast)
18538804(total leaf)
17407188(cotyledon)
17293592(total seedlings û light and dark grown)
17644812(plasma membrane - suspension cells)
17432890(leaf total membranes)
18633119(young leaf)
18431481(chloroplast)
19452453(14-3-3-interacting proteins)
18814325(cotyledons)
19200160(flower-stage 12)
19546170(mature pollen grains)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19816138(rosette leaves)
19334764(plasma membrane (cell culture))
19714877(interactomes 14-3-3 complexes (cell culture))
19888209(80S polysomal fraction)
19888209(leaves)
19376835(leaf phosphoproteins)
20340159(chloroplast heparin-binding proteins)
20706207(cell cycle interactome (cell culture))
19423572(leaf (wt and clpr2-1))
21533090(plasmodesmata-crude)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
20733066(phosphoproteome-ABA-leaf)
21173025(root proteome)
21768351(Leaf-Phosphoproteome)
21988472(vacuole (unfiltered data))

Comparative Proteomics Data

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