PPDB

The Plant Proteome Database

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AT4G05160.1   AMP-dependent synthetase and ligase family protein

Lab Annot. At4CL6 - 4-coumarate--CoA ligase
Mapman: 16.2 secondary metabolism.phenylpropanoids
Curated Location peroxisome

TAIR curated location: peroxisome15677481
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G20510.1 11Arabidopsis thaliana9E-152548426376 (16)C
AT1G20500.1 11Arabidopsis thaliana1E-14654843620 (0)C
AT5G38120.1 11Arabidopsis thaliana3E-14254341621 (1)C
Os03g05780.1 7Oryza sativa054557748 (8)C
Os03g04000.1 7Oryza sativa4E-14454941627 (7)_
Os02g46970.1 7Oryza sativa2E-12754141590 (0)_
GRMZM2G174574_P02 3.21Zea mays054258729 (0)_
v1_GRMZM2G122787_P03 4a53Zea mays054858734 (4)_
v1_GRMZM2G122787_P02 4a53Zea mays054858734 (4)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: AMP-binding(1) AMP-binding_C(1)
TargetP: Cytoplasm (Class 5 C0.099; M0.413; S0.044; _0.507)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon: 2 TMs
TAT position:
Length 544 aa (-cTP 478)
Molecular Weight 59.85 kDA(-cTP )
PI 8.59(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1518Detail       leaf [A. thaliana] wild-type
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1542Detail       leaf [A. thaliana] wild-type
2004Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2028Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2269Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2311Detail       leaf [A. thaliana] PG plastoglobule 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 

* For details about the exprimental sources click here.

Published Proteomics Data

17951448(peroxisome (leaves))
18931141(peroxisomes- high&low purity (cell culture))
18931141(peroxisomes-high purity (cell culture))
19200160(flower-stage 12)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
19329564(peroxisomes (leaf))
21311031(mitochondria)
21841088(mitochondria (2DE native gel))
21173025(root proteome)
21433285(Crude microsomes cell_cultures)

Comparative Proteomics Data


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