PPDB

 The Plant Proteome Database

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AT4G03560.1   two-pore channel 1
Lab Annot. ATTPC1 (TWO-PORE CHANNEL 1); calcium channel/ voltage-gated calcium channel
Mapman: 34.21 transport.calcium
Curated Location vacuole (Pubmed: 15772667 )

TAIR curated location: vacuole (sensu Magnoliophyta) & plasma membrane11577183,15772667
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
Os01g48680.1 7Oryza sativa070961780 (0)_
Os01g48680.2 7Oryza sativa070961780 (0)_
Zm00001eb360320_P001 agpv5Zea mays071160780 (0)_
Zm00001eb360320_P003 agpv5Zea mays057962780 (0)S
Zm00001eb360320_P002 agpv5Zea mays057962780 (0)S
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: DUF3624(1) DUF4834(2) EF-hand_1(2) EF-hand_6(2) EF-hand_7(1) EF-hand_8(2) Ion_tr
TargetP: Cytoplasm (Class 2 C0.039; M0.250; S0.020; _0.940)
Predotar:
Subcel. Location:
TM-HMM prediction: 11 TMs
Aramemnon: 12 TMs
TAT position:
Length 733 aa (-cTP 700)
Molecular Weight 84.87 kDA(-cTP )
PI 4.89(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
1260Detail       leaf [A. thaliana] co-IP SHOOT1 (rep. 1) thylakoid membranewild-type
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1611Detail       leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1614Detail       Natural Senescence leaf [A. thaliana]  
1615Detail       Natural Senescence leaf [A. thaliana]  
1744Detail        [A. thaliana] Mep-6
1745Detail        [A. thaliana] Col-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
2013Detail       leaf [A. thaliana] total leaf tissue wt
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2251Detail       seedlings [A. thaliana] total tissue wild-type
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 

* For details about the exprimental sources click here.

Published Proteomics Data

 14760709(Vacuolar proteome)
15539469(vacuole)
17151019(vacuole - suspension cell)
18931141(peroxisomes- high&low purity (cell culture))
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
21325567(root-proteome (wt & det3))
21693672(wound-regulated leaf proteome (wt))
20733066(phosphoproteome-ABA-leaf)
21173025(root proteome)
22430844(Golgi-cell-culture)
21988472(vacuole (unfiltered data))

Comparative Proteomics Data

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