PPDB

The Plant Proteome Database

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AT3G62910.1   Peptide chain release factor 1

Lab Annot. ribosome peptide chain release factor, AtPRF1 (APG3)
Mapman: 29.2.5 protein.synthesis.release
Curated Location plastid (Pubmed: 23198870 17450416 18431481 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G47020.4 11Arabidopsis thaliana9E-7733838610 (0)_
AT2G47020.1 11Arabidopsis thaliana3E-7633838610 (0)M
AT2G47020.3 11Arabidopsis thaliana6E-7336435590 (0)M
Os11g43600.1 7Oryza sativa0369769010 (10)C
Os05g19630.1 7Oryza sativa7E-7728742650 (0)_
Os05g19630.2 7Oryza sativa7E-7728742650 (0)_
Zm00001eb165700_P002 agpv5Zea mays040171830 (0)C
v1_GRMZM2G070723_P02 4a53Zea mays0401718346 (46)C
GRMZM2G070723_P02 3.21Zea mays04017183128 (54)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: ABC_tran_CTD(3) DASH_Spc34(2) PCRF(2) RF-1(1)
TargetP: Chloroplast (Class 2 C0.907; M0.291; S0.003; _0.016)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 422 aa (-cTP 372)
Molecular Weight 47.53 kDA(-cTP 41.91)
PI 7.59(-cTP 5.49)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
2013Detail       leaf [A. thaliana] total leaf tissue wt
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2115Detail       leaf [A. thaliana] Stroma stromacGep
2301Detail        [A. thaliana] Clp C1 TRAP
2310Detail       leaf [A. thaliana] PG plastoglobule 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2319Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

16358359(cell suspension (Gamborg))
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)

Comparative Proteomics Data


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