PPDB

The Plant Proteome Database

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AT3G55360.1   3-oxo-5-alpha-steroid 4-dehydrogenase family protein

Lab Annot. 3-oxo-5-alpha-steroid 4-dehydrogenase/ fatty acid elongase/ trans-2-enoyl-CoA reductase (NADPH) ATTSC13/CER10/ECR/TSC13 (ENOYL-COA REDUCTASE);
Mapman: 11.8.7 lipid metabolism.exotics (steroids, squalene etc).trans-2-enoyl-CoA reductase (NADPH)
Curated Location
TAIR curated location: fatty acid elongase complex & endoplasmic reticulum membrane15829606
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G16010.1 11Arabidopsis thaliana2E-1413033506 (6)_
Os01g05670.1 7Oryza sativa0310799062 (62)_
Os07g06800.1 7Oryza sativa7E-1016031460 (0)S
Os01g63260.1 7Oryza sativa3E-078241530 (0)_
v1_GRMZM2G481843_P01 4a53Zea mays0310829027 (27)C
GRMZM2G481843_P01 3.21Zea mays0310829028 (28)C
Zm00001eb335900_P001 agpv5Zea mays031082900 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Steroid_dh(2)
TargetP: Cytoplasm (Class 5 C0.038; M0.333; S0.100; _0.486)
Predotar:
Subcel. Location:
TM-HMM prediction: 6 TMs
Aramemnon: 4 TMs
TAT position:
Length 310 aa (-cTP 249)
Molecular Weight 35.72 kDA(-cTP )
PI 9.49(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1612Detail       Natural Senescence leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2236Detail       seedlings [A. thaliana] total tissue gfs9
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2255Detail       leaf [A. thaliana] total seedlings gfs9
2268Detail       seedlings [A. thaliana] total tissue wild-type
2271Detail       seedlings [A. thaliana] total tissue wild-type
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

16618929(ER-LOPIT-callus)
17317660(Plasma Membrane proteome)
19200160(flower-stage 12)
19546170(mature pollen grains)
19334764(plasma membrane (cell culture))
20166762(guard cells (Arabidopsis))
19114538(guard cells Arabidopsis leaf)
22060019(phosphoproteome - N-starved seedlings)
21173025(root proteome)
21988472(vacuole (unfiltered data))

Comparative Proteomics Data


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