PPDB

The Plant Proteome Database

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AT3G53110.1   P-loop containing nucleoside triphosphate hydrolases superfamily protein

Lab Annot. DEAD box RNA helicase (RH38) also LOS4 (mRNA export) dual localization cytosol & nucleus
Mapman: 27.5* RNA.DEAD/DEAH BOX helicase
Curated Location cytosol (Pubmed: 15598798 12165570 )
nucleus (Pubmed: 15598798 12165570 )

TAIR curated location: nuclear envelope & cytoplasm15598798
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G19760.1 11Arabidopsis thaliana9E-724033456160 (66)_
AT1G72730.1 11Arabidopsis thaliana5E-703883558400 (278)_
AT3G13920.1 11Arabidopsis thaliana2E-6841434561060 (673)_
Os03g06220.1 7Oryza sativa0499556924 (24)_
Os01g45190.1 7Oryza sativa3E-70386355513 (13)_
Os03g36930.1 7Oryza sativa6E-70386345513 (13)_
Zm00001eb004400_P002 agpv5Zea mays045658750 (0)_
GRMZM2G000823_P01 3.21Zea mays2E-152403557157 (8)_
Zm00001eb004400_P003 agpv5Zea mays3E-14937057740 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: AAA_19(2) DEAD(2) Helicase_C(1) ResIII(2)
TargetP: Cytoplasm (Class 4 C0.384; M0.044; S0.161; _0.783)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 496 aa (-cTP 475)
Molecular Weight 55.38 kDA(-cTP )
PI 5.18(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2321Detail       leaf [A. thaliana]  
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

17317660(Plasma Membrane proteome)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
19888209(80S polysomal fraction)
19114538(guard cells Arabidopsis leaf)
21166475(cytosolic-fractions-crude)
21173025(root proteome)

Comparative Proteomics Data


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