PPDB

The Plant Proteome Database

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AT3G52200.1   Dihydrolipoamide acetyltransferase%2C long form protein

Lab Annot. E2 dihydrolipoamide acetyltransferase- subunit of pyruvate dehydrogenase complex
Mapman: 8.1.1.2 TCA / org.transformation.TCA.pyruvate DH.E2
Curated Location mitochondria (Pubmed: 7890655 )

TAIR curated location: mitochondrion14671022,14764908,12492832
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G52200.2 11Arabidopsis thaliana0557100100105 (52)M
AT1G54220.1 11Arabidopsis thaliana6E-106456425953 (13)M
AT1G54220.2 11Arabidopsis thaliana6E-106456425952 (12)M
Os06g30460.5 7Oryza sativa0438667818 (18)_
Os06g30460.4 7Oryza sativa0438667818 (18)_
Os06g30460.3 7Oryza sativa0438667818 (18)_
Zm00001eb061570_P001 agpv5Zea mays043968820 (0)M
GRMZM2G082664_P01 3.21Zea mays0439688229 (21)M
v1_GRMZM2G082664_P01 4a53Zea mays5E-122223778818 (18)S
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: 2-oxoacid_dh(1) ATP-synt_ab_Xtn(2) Biotin_lipoyl(2) Biotin_lipoyl_2(2) DUF2118(2
TargetP: Mitochondria (Class 1 C0.061; M0.874; S0.006; _0.016)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 637 aa (-cTP 629)
Molecular Weight 68.86 kDA(-cTP )
PI 5.38(-cTP )

Experimental Evidence

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View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1535Detail       leaf [A. thaliana] wild-type
1538Detail       leaf [A. thaliana] plastid RNCs thylakoid membranewild-type
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2269Detail       seedlings [A. thaliana] total tissue wild-type
2271Detail       seedlings [A. thaliana] total tissue wild-type
2273Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2290Detail        [A. thaliana] Clp C1 TRAP
2297Detail        [A. thaliana] Clp C1 WT
2300Detail        [A. thaliana] Clp C1 TRAP
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2398Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/35SRecA-GFP
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

MitoDB(Mitochondrial proteome)
14764908(mitochondria-suspension cells)
17293592(total seedlings û light and dark grown)
17137349(ATP-binding-mitochondria cell suspension)
18385124(mitochondria - leaf and suspension cell)
18385124(mitochondria)
18931141(peroxisomes- high&low purity (cell culture))
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
19888209(leaves)
19114538(guard cells Arabidopsis leaf)
22060019(phosphoproteome - N-starved seedlings)
21166475(cytosolic-fractions-crude)
21841088(mitochondria (2DE native gel))
21173025(root proteome)
21472856(mitochondrial-2DEgels-uncurated)
21472856(mitochondria-LC-MS/MS-uncurated)
21472856(mitochondrial-reference-proteome-Millar-Nov2011)
21433285(Crude microsomes cell_cultures)
21515685(GreenCut2)

Comparative Proteomics Data


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