PPDB

 The Plant Proteome Database

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AT3G51260.2   20S proteasome alpha subunit PAD1
Lab Annot. 20S proteasome alpha subunit D (PAD1)
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: nucleus & proteasome core complex (sensu Eukaryota) & spindle & phragmoplast9373170,9611183,11387208
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G51260.1 11Arabidopsis thaliana2E-1772509797267 (153)_
AT5G66140.1 11Arabidopsis thaliana1E-1602509396122 (68)_
AT3G22110.1 11Arabidopsis thaliana8E-542004365268 (155)M
Os08g43540.1 7Oryza sativa6E-148225889334 (34)S
Os09g36710.1 7Oryza sativa1E-147225889320 (20)_
Os06g07878.1 7Oryza sativa3E-55247406115 (15)M
Zm00001eb202760_P001 agpv5Zea mays4E-14922589930 (0)_
GRMZM2G050467_P01 3.21Zea mays4E-149225899346 (33)_
v1_GRMZM2G050467_P01 4a53Zea mays4E-149225899348 (47)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Proteasome(1) Proteasome_A_N(1)
TargetP: Cytoplasm (Class 3 C0.010; M0.400; S0.068; _0.813)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 243 aa (-cTP 199)
Molecular Weight 26.57 kDA(-cTP )
PI 6.86(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
541Detail       leaf [A. thaliana] stroma (FPLC) >1 MDa stromawild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2193Detail        [A. thaliana] total soluble P3-strepII 26
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2222Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2253Detail       seedlings [A. thaliana] total tissue atg5
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2297Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP

* For details about the exprimental sources click here.

Published Proteomics Data

 15821981(crude&pure 80S ribosome)
17151019(vacuole - suspension cell)
19200160(flower-stage 12)
20118269(developing seeds)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
19423572(leaf (wt and clpr2-1))
20516081(tagged-26S proteasome-affinity)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data

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