PPDB

The Plant Proteome Database

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AT3G46100.1   Histidyl-tRNA synthetase 1

Lab Annot. His-tRNA synthetase (ATHRS1) / histidine--tRNA ligase (dual targeted)
Mapman: 29.1.21 protein.aa activation.histidine-tRNA ligase
Curated Location mitochondria (Pubmed: 23198870 16251277 )
plastid stroma (Pubmed: 23198870 16251277 )

TAIR curated location: mitochondrion & chloroplast9684861,16251277
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G03406.1 11Arabidopsis thaliana3E-16826588920 (0)M
AT3G02760.1 11Arabidopsis thaliana2E-384252948134 (73)C
AT3G02760.2 11Arabidopsis thaliana2E-3842529480 (0)C
Os02g51830.1 7Oryza sativa0460648111 (11)M
Os05g05840.1 7Oryza sativa1E-43428315137 (37)_
Zm00001eb122020_P001 agpv5Zea mays042867850 (0)M
v1_GRMZM2G158228_P01 4a53Zea mays0428678531 (31)M
GRMZM2G158228_P01 3.21Zea mays0428678571 (43)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: HGTP_anticodon(1) tRNA-synt_2b(1) tRNA-synt_2d(1) tRNA-synt_His(1)
TargetP: Mitochondria (Class 4 C0.220; M0.594; S0.061; _0.060)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 486 aa (-cTP 445)
Molecular Weight 54.56 kDA(-cTP )
PI 6.94(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
324Detail       leaf [A. thaliana] cell wall enriched wild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
541Detail       leaf [A. thaliana] stroma (FPLC) >1 MDa stromawild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1935Detail       leaf [A. thaliana] chloroplast wild-type
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2112Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2300Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2320Detail        [A. thaliana] Thylakoids plastoglobule 

* For details about the exprimental sources click here.

Published Proteomics Data

18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
19995723(chloroplast reference proteome)
21841088(mitochondria (2DE native gel))

Comparative Proteomics Data


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