PPDB

 The Plant Proteome Database

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AT3G44300.1   nitrilase 2
Lab Annot. nitrilase 2 (NIT2)
Mapman: 17.2.1 hormone metabolism.auxin.synthesis-degradation
Curated Location
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G44310.1 11Arabidopsis thaliana034090951500 (1202)_
AT3G44310.3 11Arabidopsis thaliana034090951414 (1116)_
AT3G44320.1 11Arabidopsis thaliana0340839228 (25)_
Os02g42350.1 7Oryza sativa2E-16333965790 (0)_
Os02g42330.1 7Oryza sativa1E-15431564792 (2)_
Os02g42330.2 7Oryza sativa2E-11623666792 (2)_
Zm00001eb185550_P001 agpv5Zea mays1E-16132365790 (0)_
v1_GRMZM2G111225_P01 4a53Zea mays1E-16132365790 (0)_
v1_GRMZM2G111225_P02 4a53Zea mays1E-16132365790 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: CN_hydrolase(1)
TargetP: Cytoplasm (Class 4 C0.285; M0.061; S0.241; _0.517)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 339 aa (-cTP 320)
Molecular Weight 37.15 kDA(-cTP )
PI 5.35(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1091Detail        [A. thaliana] R4Strep Complemented
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1650Detail       leaf [A. thaliana] plastoglobules plastoglobulem48
2004Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2208Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII
2246Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2262Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2264Detail       leaf [A. thaliana] stroma Clp stromawild-type
2284Detail        [A. thaliana] Clp C1 WT
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 16358359(cell suspension (Gamborg))
16358359(cell suspension (M&Skoog))
16502469(cell suspension-Cd-up)
16502469(cell suspension-CD-down)
17407188(cotyledon)
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
19714877(interactomes 14-3-3 complexes (cell culture))
19888209(leaves)
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)
21472856(mitochondrial-2DEgels-uncurated)
21433285(Crude microsomes cell_cultures)
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data

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