PPDB

The Plant Proteome Database

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AT3G27430.1   N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein

Lab Annot. 20S proteasome beta subunit B (PBB1)
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: proteasome core complex (sensu Eukaryota)9373170,9611183
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G27430.2 11Arabidopsis thaliana024810010070 (70)_
AT5G40580.2 11Arabidopsis thaliana02489710021 (21)_
AT5G40580.1 11Arabidopsis thaliana02489710021 (21)_
Os05g09490.2 7Oryza sativa7E-159240869443 (43)_
Os05g09490.1 7Oryza sativa7E-159240869443 (43)_
Os02g53060.1 7Oryza sativa2E-25182325439 (39)_
Zm00001eb304090_P003 agpv5Zea mays9E-15924285930 (0)_
Zm00001eb304090_P001 agpv5Zea mays9E-15924285930 (0)_
v1_GRMZM2G074549_P03 4a53Zea mays9E-159242859314 (14)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Proteasome(1)
TargetP: Cytoplasm (Class 1 C0.108; M0.102; S0.102; _0.917)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 267 aa (-cTP 246)
Molecular Weight 28.81 kDA(-cTP )
PI 8.94(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2043Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2051Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2052Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2053Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2245Detail       seedlings [A. thaliana] total tissue wild-type
2248Detail       seedlings [A. thaliana] total tissue wild-type

* For details about the exprimental sources click here.

Published Proteomics Data

16055689(Glutathionylated suspension cell)
19546170(mature pollen grains)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
19888209(leaves)
20516081(tagged-26S proteasome-affinity)
21325567(root-proteome (wt & det3))
21173025(root proteome)

Comparative Proteomics Data


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