PPDB

 The Plant Proteome Database

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AT3G25660.1   Amidase family protein
Lab Annot. Glu-tRNA(Gln) amidotransferase subunit A (GATA or GLU-ADT subunit A) - dual targeted mito&chloro
Mapman: 29.1.50* protein.aa activation.glutamate-tRNA amidotransferase
Curated Location mitochondria (Pubmed: 23198870 18441100 )
plastid stroma (Pubmed: 23198870 18441100 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G64440.1 11Arabidopsis thaliana7E-44476284424 (19)_
AT5G07360.1 11Arabidopsis thaliana2E-2723134505 (0)M
AT5G07360.3 11Arabidopsis thaliana2E-2723134500 (0)M
Os04g55050.1 7Oryza sativa0450678146 (46)M
Os04g55050.2 7Oryza sativa0390688336 (36)M
Os12g07150.1 7Oryza sativa2E-4352930476 (6)_
Zm00001eb068770_P001 agpv5Zea mays041068812 (2)M
GRMZM2G022192_P03 3.21Zea mays0410688179 (47)M
v1_GRMZM2G022192_P01 4a53Zea mays0410688136 (36)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Amidase(1)
TargetP: Chloroplast (Class 4 C0.768; M0.467; S0.001; _0.022)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 537 aa (-cTP 487)
Molecular Weight 57.18 kDA(-cTP 51.73)
PI 6.16(-cTP 5.47)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
151Detail       leaf [A. thaliana] chloroplast stromawild-type
303Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
1127Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1456Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1826Detail       leaf tip mesophyll [Zea Mays] chloroplast stromaW22 (repl. 3)
1871Detail       Total leaf [A. thaliana] Stroma wild-type
1934Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2034Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2039Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2040Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2041Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2042Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2043Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2044Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2045Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2046Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2047Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2051Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2055Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2057Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2059Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2061Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2063Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2065Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2067Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2069Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2071Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2073Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2074Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2112Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2141Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2142Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2143Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2144Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2145Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2146Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2147Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2148Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2315Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

 16207701(chloroplast stroma)
18431481(chloroplast)
20423899(chloroplast)
19888209(leaves)
19995723(chloroplast reference proteome)
21311031(mitochondria)
21173025(root proteome)

Comparative Proteomics Data

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