PPDB

 The Plant Proteome Database

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AT3G22960.1   Pyruvate kinase family protein
Lab Annot. pyruvate kinase-1 (typically homotetramer)
Mapman: 11.1.30 lipid metabolism.FA synthesis and FA elongation.pyruvate kinase
Curated Location plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G52920.1 11Arabidopsis thaliana2E-1264754561432 (294)C
AT1G32440.1 11Arabidopsis thaliana2E-123482446266 (35)C
AT5G63680.1 11Arabidopsis thaliana9E-74517335269 (23)_
Os07g08340.1 7Oryza sativa0499859539 (39)C
Os07g08340.2 7Oryza sativa0454869535 (35)_
Os03g46910.1 7Oryza sativa050460750 (0)C
GRMZM2G066290_P02 3.21Zea mays0454869526 (25)M
v1_GRMZM2G066290_P02 4a53Zea mays0454869517 (17)M
Zm00001eb060760_P002 agpv5Zea mays035287950 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: HpcH_HpaI(1) PK(1) PK_C(1)
TargetP: Chloroplast (Class 2 C0.862; M0.211; S0.002; _0.025)
Predotar:
Subcel. Location: Lumen
TM-HMM prediction: No
Aramemnon:
TAT position: 117
Length 596 aa (-cTP 549) (-lTP 457)
Molecular Weight 65.13 kDA(-cTP 59.65)(-lTP 50.29)
PI 5.64(-cTP 5.11)(-lTP 5.352)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
99Detail       petal [B. oleracea] plastid stroma 
119Detail       root [B. rapa] plastid stroma 
135Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
151Detail       leaf [A. thaliana] chloroplast stromawild-type
303Detail       leaf [A. thaliana] chloroplast stromawild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
412Detail       leaf [A. thaliana] stroma - R-X102 tag-CNPAGE clpr2-1-TapTagR2
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
617Detail       leaf [A. thaliana] chloroplast stromawild-type
618Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
620Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
641Detail        [A. thaliana] plastid nucleoid (crude) wild-type
698Detail       leaf [A. thaliana] stroma- QconCAT wild-type
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1176Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1215Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1456Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1517Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1597Detail        [A. thaliana]  
1749Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1870Detail       Total leaf [A. thaliana] Stroma wild-type
1871Detail       Total leaf [A. thaliana] Stroma wild-type
1872Detail       Total leaf [A. thaliana] Stroma wild-type
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1933Detail       leaf [A. thaliana] chloroplast wild-type
1934Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
1999Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2000Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2001Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2002Detail       leaf [A. thaliana] stroma ClpS stromaclpS1
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2039Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2040Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2041Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2042Detail       Total leaf [A. thaliana] chloroplast stromawt and icp55c-3
2047Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2051Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2052Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2055Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2056Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2057Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2059Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2061Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2063Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2065Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2066Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2067Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2068Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2069Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2072Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2073Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2074Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2076Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2083Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2117Detail       leaf [A. thaliana] Stroma stromacGep
2141Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2142Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2143Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2144Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2145Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2146Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2147Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2148Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2149Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2150Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2151Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2152Detail       Total leaf [A. thaliana] total soluble stromawt & cgep
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2269Detail       seedlings [A. thaliana] total tissue wild-type
2271Detail       seedlings [A. thaliana] total tissue wild-type
2273Detail       seedlings [A. thaliana] total tissue wild-type
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2315Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
16207701(chloroplast stroma)
16358359(cell suspension (Gamborg))
16502469(cell suspension-Cd-up)
15052571(soluble leaf)
18538804(total leaf)
18431481(chloroplast)
19200160(flower-stage 12)
19546170(mature pollen grains)
20118269(developing seeds)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
20423899(chloroplast)
19888209(leaves)
20166762(guard cells (Arabidopsis))
19114538(guard cells Arabidopsis leaf)
19423572(leaf (wt and clpr2-1))
19423572(stroma chloroplast (wt and clpr2-1))
19995723(chloroplast reference proteome)
21311031(chloroplasts)
21311031(mitochondria)
21166475(cytosolic-fractions-crude)
21173025(root proteome)
21433285(Crude microsomes cell_cultures)
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
180_181clpr2-1 cICAT - exp1 / wt cICAT - exp1cICAT3ISEEICNSAAK + ICAT_l / ISEEICNSAAK + ICAT_h21.98 leaf [A. thaliana] chloroplast stroma

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