PPDB

 The Plant Proteome Database

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AT3G20330.1   PYRIMIDINE B
Lab Annot. chloroplast aspartate transcarbamylase / ATCase (PYRB)
Mapman: 23.1.1.2 nucleotide metabolism.synthesis.pyrimidine.aspartate transcarbamoylase
Curated Location plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G20330.2 11Arabidopsis thaliana03901001000 (0)C
AT1G75330.1 11Arabidopsis thaliana7E-213132946218 (157)C
Os08g15030.1 7Oryza sativa031381922 (2)M
Os02g47590.1 7Oryza sativa2E-24311304633 (33)C
Zm00001eb412930_P003 agpv5Zea mays036572840 (0)M
GRMZM2G101900_P02 3.21Zea mays0365728413 (12)M
GRMZM2G101900_P01 3.21Zea mays0365728413 (12)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: DELLA(2) OTCace(1) OTCace_N(1)
TargetP: Chloroplast (Class 2 C0.914; M0.011; S0.270; _0.018)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 390 aa (-cTP 322)
Molecular Weight 43.17 kDA(-cTP 36.04)
PI 6.21(-cTP 5.51)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
120Detail       root [B. rapa] plastid stroma 
151Detail       leaf [A. thaliana] chloroplast stromawild-type
303Detail       leaf [A. thaliana] chloroplast stromawild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
618Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
641Detail        [A. thaliana] plastid nucleoid (crude) wild-type
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1514Detail       leaf [A. thaliana] wild-type
1517Detail       leaf [A. thaliana] wild-type
1521Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1700Detail        [A. thaliana] Col-0
1703Detail        [A. thaliana] cGep
1870Detail       Total leaf [A. thaliana] Stroma wild-type
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1933Detail       leaf [A. thaliana] chloroplast wild-type
1934Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2311Detail       leaf [A. thaliana] PG plastoglobule 
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6

* For details about the exprimental sources click here.

Published Proteomics Data

 16207701(chloroplast stroma)
15052571(soluble leaf)
17828791(total leaf)
18431481(chloroplast)
19452453(14-3-3-interacting proteins)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
19888209(leaves)
19995723(chloroplast reference proteome)
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
195_194ffc1-2 cICAT- exp1 / wt cICAT- exp1cICAT8CDVVYQTR + ICAT_l / CDVVYQTR + ICAT_h20.87 leaf [A. thaliana] chloroplast stroma

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