PPDB

 The Plant Proteome Database

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AT3G14290.1   20S proteasome alpha subunit E2
Lab Annot. 20S proteasome alpha subunit E2 (PAE2)
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: proteasome core complex (sensu Eukaryota)9373170,9611183
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G53850.1 11Arabidopsis thaliana1E-17623798100175 (105)_
AT1G53850.2 11Arabidopsis thaliana1E-17623798100162 (92)_
AT3G22110.1 11Arabidopsis thaliana5E-562414163245 (155)M
Os11g40140.1 7Oryza sativa2E-170237949969 (69)_
Os06g07140.1 7Oryza sativa2E-50217416219 (19)M
Os06g07878.1 7Oryza sativa2E-50217416215 (15)M
GRMZM2G056870_P01 3.21Zea mays1E-170237949962 (56)_
v1_GRMZM2G056870_P01 4a53Zea mays1E-170237949960 (59)_
Zm00001eb004030_P001 agpv5Zea mays1E-17023794990 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Proteasome(1) Proteasome_A_N(1)
TargetP: Cytoplasm (Class 3 C0.044; M0.318; S0.048; _0.837)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 237 aa (-cTP 232)
Molecular Weight 25.98 kDA(-cTP )
PI 4.70(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
135Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1581Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1596Detail        [A. thaliana]  
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2245Detail       seedlings [A. thaliana] total tissue wild-type
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2287Detail        [A. thaliana] Clp C1 WT
2296Detail        [A. thaliana] Clp C1 WT
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2315Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 15821981(crude&pure 80S ribosome)
15734919(80S cytolic ribosomes)
16055689(Glutathionylated suspension cell)
15815986(seedling)
15815986(Leaf)
11402211,12068122,15047896,16679420,17028149(seed-all-Jobpapers)
18599647(seed proteome)
18814325(cotyledons)
19200160(flower-stage 12)
19546170(mature pollen grains)
20118269(developing seeds)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
20516081(tagged-26S proteasome-affinity)
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data

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