PPDB

PPDB	The Plant Proteome Database

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AT3G10270.1 DNA gyrase subunit B

Lab Annot.

DNA gyrase B1 - dual targeted plastids and mitochondria
Mapman: 28.2 DNA.repair

Curated Location

mitochondria (Pubmed: 23198870 15136745 )
plastid nucleoid (Pubmed: 23198870 15136745 )

TAIR curated location: mitochondrion & cytoplasm14671022,16169894

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT3G10270.2 11 Arabidopsis thaliana 0 730 100 100 0 (0) M
AT5G04130.1 11 Arabidopsis thaliana 0 737 86 91 29 (13) M
AT5G04130.2 11 Arabidopsis thaliana 0 521 83 90 2 (1) M
Os01g16290.1 7 Oryza sativa 0 668 77 89 18 (18) C
Os01g16290.2 7 Oryza sativa 0 549 77 89 18 (18) C
Os02g47150.1 7 Oryza sativa 6E-29 577 25 43 2 (2) _
Zm00001eb128720_P003 agpv5 Zea mays 0 703 75 85 0 (0) C
v1_GRMZM2G111014_P01 4a53 Zea mays 0 703 75 85 52 (52) C
Zm00001eb128720_P001 agpv5 Zea mays 0 703 75 85 0 (0) C
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	DNA_gyraseB(1) DNA_gyraseB_C(1) HATPase_c(1) HATPase_c_3(1) Toprim(1)
TargetP:	Mitochondria (Class 3 C0.388; M0.853; S0.001; _0.003)
Predotar:
Subcel. Location:
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	730 aa (-cTP 667)
Molecular Weight	81.05 kDA(-cTP )
PI	8.63(-cTP )

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
542	Detail								leaf [A. thaliana]	stroma (FPLC) >500 kDa (HMB)	wild-type
640	Detail								leaf [A. thaliana]	plastid nucleoid	wild-type
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1418	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 3)
1426	Detail								leaf [A. thaliana]	total leaf tissue	prep1xprep2xt1xt2 (rep. 3)
1453	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 1)
1454	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 2)
1525	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 1)
1528	Detail								leaf [A. thaliana]	chloroplast nucleoid	wild-type (rep. 1)
1638	Detail								leaf [A. thaliana]	total leaf tissue stroma	Wild-type
1643	Detail								leaf [A. thaliana]	total leaf tissue	Wild-type
2289	Detail								[A. thaliana]		Clp C1 TRAP
2291	Detail								[A. thaliana]		Clp C1 TRAP
2296	Detail								[A. thaliana]		Clp C1 WT
2303	Detail								[A. thaliana]		Clp C1 WT
2305	Detail								[A. thaliana]		Clp C1 TRAP

* For details about the exprimental sources click here.

Published Proteomics Data

MitoDB(Mitochondrial proteome)
16326926(nucleoids)
19525416(leaf (wt and clpr4-1 mutant))
21311031(mitochondria)
21173025(root proteome)
21472856(mitochondrial-reference-proteome-Millar-Nov2011)

Comparative Proteomics Data