|
AT3G02660.1
Tyrosyl-tRNA synthetase%2C class Ib%2C bacterial/mitochondrial
|
| Lab Annot. |
tRNA synthetase class I (W and Y) (dual targeted- cTP/mTP) Mapman: 29.1.1 protein.aa activation.tyrosine-tRNA ligase |
| Curated Location |
mitochondria (Pubmed: 23198870 16251277 ) plastid stroma (Pubmed: 23198870 16251277 )
TAIR curated location: mitochondrion & chloroplast16251277 |
| Species |
Arabidopsis thaliana
Source:
TAIR Arabidopsis (v.11) |
| Links |
TAIR PeptideAtlas POGS SUBA Uniprot PTM
Get sequence
|
Related Genes
|
#Spots: The number of publicly accessible spots are in parenthesis
|
| Prediction |
| PFAM: |
S4(1) tRNA-synt_1b(1) TyrRSs_C(1) |
| TargetP: |
Chloroplast (Class 2 C0.860; M0.191; S0.004; _0.003) |
| Predotar: |
|
| Subcel. Location: |
Stroma |
| TM-HMM prediction: |
No |
| Aramemnon: |
|
| TAT position: |
|
| Length |
511 aa (-cTP 447) |
| Molecular Weight |
56.62 kDA(-cTP 49.56) |
| PI |
6.02(-cTP 5.29) |
|
|
Experimental Evidence
Expand
View Identified Peptides
View GeneModel
|
| Expr* | Spot | MW native | MW denatured | pI native | pI denatured | Type | Mowse | Ambiguity | Tissue | Sample from | Genotype |
| 180_181 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpr2-1 |
| 326 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type |
| 339 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type |
| 361 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 | wild-type |
| 371 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | clpr4-1 |
| 412 | Detail | | | | | | | | leaf [A. thaliana] | stroma - R-X102 tag-CNPAGE | clpr2-1-TapTagR2 |
| 527 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wild-type (wt1) |
| 528 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wild-type (wt2) |
| 544 | Detail | | | | | | | | leaf [A. thaliana] | stroma (FPLC) <600 kDa (LMA) | wild-type |
| 621 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | clpr4-1 |
| 622 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wild-type |
| 873 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type |
| 874 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type |
| 1207 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | clpp3 null |
| 1208 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | clpp3 null |
| 1392 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wild-type (rep. 1) |
| 1396 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | clpt1xclpt2 (rep. 2) |
| 1399 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.14 -rep. 1 | clpp3-1 |
| 1400 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.14 -rep. 1 | wild-type |
| 1401 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.14 -rep. 1 | clpp3-1 |
| 1403 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.14 -rep. 1 | clpp3-1 |
| 1405 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 rep 2. | wild-type |
| 1407 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 rep 2. | clpr2-1 |
| 1408 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 rep 2. | clpr2-1 |
| 1411 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 rep 1.1 | clpr2-1 |
| 1412 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue 1.07 rep 1.2 | clpr2-1 |
| 1417 | Detail | | | | | | | | leaf [A. thaliana] | total tissue (2% sucrose) | clpr4-1 (rep. 2) |
| 1418 | Detail | | | | | | | | leaf [A. thaliana] | total tissue (2% sucrose) | clpr4-1 (rep. 3) |
| 1421 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wild-type (rep. 1) |
| 1425 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | prep1xprep2xt1xt2 (rep. 2) |
| 1426 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | prep1xprep2xt1xt2 (rep. 3) |
| 1431 | Detail | | | | | | | | leaf [A. thaliana] | total tissue (2% sucrose) | wild-type (rep. 2) |
| 1453 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast nucleoid | wild-type (rep. 1) |
| 1455 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type (rep. 1) |
| 1456 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type (rep. 2) |
| 1457 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type (rep. 3) |
| 1458 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clps (rep. 1) |
| 1461 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 (rep. 1) |
| 1462 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 (rep. 2) |
| 1463 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 (rep. 3) |
| 1481 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | wild-type (rep. 1) |
| 1482 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 x clps (rep. 1) |
| 1484 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 x clps (rep. 2) |
| 1486 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast stroma | clpc1 x clps (rep. 3) |
| 1872 | Detail | | | | | | | | Total leaf [A. thaliana] | Stroma | wild-type |
| 1933 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast | wild-type |
| 1936 | Detail | | | | | | | | leaf [A. thaliana] | chloroplast | Prep1_Prep2 |
| 1941 | Detail | | | | | | | | leaf [A. thaliana] | stroma WT stroma | wild-type |
| 1942 | Detail | | | | | | | | leaf [A. thaliana] | stroma WT stroma | wild-type |
| 1943 | Detail | | | | | | | | leaf [A. thaliana] | stroma WT stroma | wild-type |
| 1944 | Detail | | | | | | | | leaf [A. thaliana] | stroma UVR stroma | UVR |
| 1945 | Detail | | | | | | | | leaf [A. thaliana] | stroma UVR stroma | UVR |
| 1947 | Detail | | | | | | | | leaf [A. thaliana] | stroma ClpS stroma | clpS |
| 1948 | Detail | | | | | | | | leaf [A. thaliana] | stroma ClpS stroma | clpS |
| 1949 | Detail | | | | | | | | leaf [A. thaliana] | stroma ClpS stroma | clpS |
| 1984 | Detail | | | | | | | | leaf [A. thaliana] | plastid | wild-type |
| 1985 | Detail | | | | | | | | leaf [A. thaliana] | plastid | wild-type |
| 1987 | Detail | | | | | | | | leaf [A. thaliana] | plastid | Prep1_Prep2 |
| 1988 | Detail | | | | | | | | leaf [A. thaliana] | plastid | Prep1_Prep2 |
| 2012 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | prep1-1xprep2-1xopda1-2 |
| 2013 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | wt |
| 2016 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | prep1-1xprep2-1xopda1-2 |
| 2020 | Detail | | | | | | | | leaf [A. thaliana] | total leaf tissue | prep1-1xprep2-1xopda1-2 |
| 2112 | Detail | | | | | | | | leaf [A. thaliana] | Stroma stroma | wt |
| 2113 | Detail | | | | | | | | leaf [A. thaliana] | Stroma stroma | wt |
| 2114 | Detail | | | | | | | | leaf [A. thaliana] | Stroma stroma | wt |
| 2115 | Detail | | | | | | | | leaf [A. thaliana] | Stroma stroma | cGep |
| 2116 | Detail | | | | | | | | leaf [A. thaliana] | Stroma stroma | cGep |
| 2143 | Detail | | | | | | | | Total leaf [A. thaliana] | total soluble stroma | wt & cgep |
| 2146 | Detail | | | | | | | | Total leaf [A. thaliana] | total soluble stroma | wt & cgep |
| 2147 | Detail | | | | | | | | Total leaf [A. thaliana] | total soluble stroma | wt & cgep |
| 2148 | Detail | | | | | | | | Total leaf [A. thaliana] | total soluble stroma | wt & cgep |
| 2150 | Detail | | | | | | | | Total leaf [A. thaliana] | total soluble stroma | wt & cgep |
| 2184 | Detail | | | | | | | | Rosettes [A. thaliana] | total soluble | tic40 and wt |
| 2185 | Detail | | | | | | | | Rosettes [A. thaliana] | total soluble | tic40 and wt |
| 2245 | Detail | | | | | | | | seedlings [A. thaliana] | total tissue | wild-type |
| 2400 | Detail | | | | | | | | leaf-soluble [A. thaliana] | GFP-nano-trap | wt/DUF760-1GFP/ClpC1TRAPSTREP |
* For details about the exprimental sources
click here.
|
|
Published Proteomics Data
|
18431481(chloroplast) 19525416(leaf (wt and clpr4-1 mutant)) 20423899(chloroplast) 19888209(leaves) 19423572(stroma chloroplast (wt and clpr2-1)) 19995723(chloroplast reference proteome) |
|
Comparative Proteomics Data
|
| Expr* | Source | Tech. | Spot | PepSeq | ChargeState(z) | AreaRatio | Ambiguity | Tissue | Sample from |
| 180_181 | clpr2-1 cICAT - exp1 / wt cICAT - exp1 | cICAT | 5 | R.IAEDIPSCSLPIDR.V + ICAT_l / R.IAEDIPSCSLPIDR.V + ICAT_h | 2 | 0.96 | | leaf [A. thaliana] | chloroplast stroma |
|