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The Plant Proteome Database

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AT3G02520.1   general regulatory factor 7

Lab Annot. 14-3-3 protein GF14 nu (grf7)
Mapman: 30.7 signalling.14-3-3 proteins
Curated Location not plastid (Pubmed: 10631267 ) (supported by in-house exprements)

TAIR curated location: nuclear envelope & plasma membrane & chloroplast stroma & cytoplasm9276953
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT3G02520.2 11Arabidopsis thaliana02651001000 (0)_
AT5G38480.1 11Arabidopsis thaliana1E-1662509096535 (310)_
AT5G38480.2 11Arabidopsis thaliana3E-1642508895427 (263)_
Os08g33370.2 7Oryza sativa2E-166256879568 (68)_
Os08g33370.1 7Oryza sativa2E-166256879568 (68)_
Os03g50290.1 7Oryza sativa3E-161254879377 (77)_
v1_GRMZM2G078641_P01 4a53Zea mays8E-1632568594130 (129)_
Zm00001eb424290_P002 agpv5Zea mays8E-16325685940 (0)_
Zm00001eb424290_P001 agpv5Zea mays8E-16325685940 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: 14-3-3(1) Orbi_VP5(1)
TargetP: Cytoplasm (Class 2 C0.174; M0.086; S0.142; _0.835)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 265 aa (-cTP 239)
Molecular Weight 29.82 kDA(-cTP )
PI 4.74(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
119Detail       root [B. rapa] plastid stroma 
294Detail       embryo [barley] affinity purified 14-3-3 colum  
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
617Detail       leaf [A. thaliana] chloroplast stromawild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1125Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1127Detail       leaf [A. thaliana] chloroplast plastoglobulek2k3
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1448Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 1)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1508Detail        [A. thaliana]  
1517Detail       leaf [A. thaliana] wild-type
1519Detail       leaf [A. thaliana] wild-type
1521Detail       leaf [A. thaliana] wild-type
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1540Detail       leaf [A. thaliana] Thylakoids plastoglobulewild-type
1541Detail       leaf [A. thaliana] Thylakoids plastoglobuleM-48
1542Detail       leaf [A. thaliana] wild-type
1581Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1596Detail        [A. thaliana]  
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1651Detail       leaf [A. thaliana] plastoglobules plastoglobulewild-type
1713Detail       Rosettes [A. thaliana] Thylakoids thylakoid membranek1k3
1745Detail        [A. thaliana] Col-0
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
2003Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2007Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2008Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2028Detail       leaf [A. thaliana] WT PG plastoglobuleWT
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2253Detail       seedlings [A. thaliana] total tissue atg5
2262Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2270Detail       seedlings [A. thaliana] total tissue wild-type
2271Detail       seedlings [A. thaliana] total tissue wild-type
2272Detail       seedlings [A. thaliana] total tissue wild-type
2284Detail        [A. thaliana] Clp C1 WT
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2300Detail        [A. thaliana] Clp C1 TRAP
2316Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2399Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1WTSTREP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

15028209(Total chloroplast)
16358359(cell suspension (Gamborg))
16619310(14-3-3 proteins)
17317660(Plasma Membrane proteome)
18538804(total leaf)
17644812(plasma membrane - suspension cells)
18814325(cotyledons)
18931141(peroxisomes- high&low purity (cell culture))
19200160(flower-stage 12)
19546170(mature pollen grains)
19260003(detergent resistant plasma membrane (DRM) proteins (Cell cultures))
19525416(leaf (wt and clpr4-1 mutant))
19816138(rosette leaves)
19334764(plasma membrane (cell culture))
19714877(interactomes 14-3-3 complexes (cell culture))
19888209(80S polysomal fraction)
19888209(leaves)
19423572(leaf (wt and clpr2-1))
21533090(plasmodesmata-crude)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21173025(root proteome)
22430844(Golgi-cell-culture)
21472856(mitochondrial-2DEgels-uncurated)

Comparative Proteomics Data


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