PPDB

PPDB	The Plant Proteome Database

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AT2G39930.1 isoamylase 1

Lab Annot.

isoamylase 1 (starch debranching enzyme) (AtlSA1)
Mapman: 2.1.2.4 major CHO metabolism.synthesis.starch.debranching

Curated Location

plastid (Pubmed: 23198870 18431481 ) (supported by in-house exprements)

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT2G39930.2 11 Arabidopsis thaliana 0 669 98 98 0 (0) C
AT2G39930.3 11 Arabidopsis thaliana 0 602 100 100 0 (0) C
AT4G09020.1 11 Arabidopsis thaliana 0 741 45 59 193 (138) C
Os08g40930.1 7 Oryza sativa 0 709 72 83 0 (0) C
Os09g29404.1 7 Oryza sativa 1E-107 406 45 59 0 (0) _
Os09g29404.3 7 Oryza sativa 1E-107 406 45 59 0 (0) _
v1_GRMZM2G150796_P01 4a53 Zea mays 0 732 45 59 3 (3) M
Zm00001eb315710_P001 agpv5 Zea mays 0 732 45 59 0 (0) M
Zm00001eb174590_P001 agpv5 Zea mays 0 710 71 83 0 (0) M
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	Alpha-amylase(3) CBM_48(3) Zn_ribbon_2(2)
TargetP:	Chloroplast (Class 3 C0.659; M0.061; S0.035; _0.232)
Predotar:
Subcel. Location:	Stroma
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	783 aa (-cTP 740)
Molecular Weight	89.48 kDA(-cTP 84.62)
PI	5.78(-cTP 5.50)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
94	Detail								root [B. rapa]	plastid stroma
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
326	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
339	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
342	Detail								leaf [A. thaliana]	chloroplast low density membrane	wild-type
542	Detail								leaf [A. thaliana]	stroma (FPLC) >500 kDa (HMB)	wild-type
869	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
873	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
874	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
877	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1175	Detail								leaf [A. thaliana]	co-IP RH3 stroma	wild-type
1178	Detail								leaf [A. thaliana]	co-IP RH3 stroma	clpr2-1
1207	Detail								leaf [A. thaliana]	total leaf tissue	clpp3 null
1214	Detail								leaf [A. thaliana]	co-IP RH3 stroma	wild-type
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1439	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 1)
1440	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 2)
1458	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 1)
1459	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 2)
1460	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 3)
1462	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 2)
1465	Detail								leaf [A. thaliana]	co-IP GST-ClpS stroma	clpc1 x clps (rep. 1)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1482	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 1)
1483	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1484	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 2)
1485	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1486	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 3)
1531	Detail								leaf [A. thaliana]	total leaf tissue - 40 uM CAP	wild-type (rep. 1)
1934	Detail								leaf [A. thaliana]	chloroplast	wild-type
1941	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1948	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1949	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1984	Detail								leaf [A. thaliana]	plastid	wild-type
1985	Detail								leaf [A. thaliana]	plastid	wild-type
1986	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1987	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1988	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
2002	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2179	Detail								leaf [A. thaliana]	stroma Clp	GST ClpF x ClpS1
2297	Detail								[A. thaliana]		Clp C1 WT
2300	Detail								[A. thaliana]		Clp C1 TRAP
2301	Detail								[A. thaliana]		Clp C1 TRAP
2307	Detail								[A. thaliana]		Clp C1 TRAP
2312	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2313	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2314	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2315	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2320	Detail								[A. thaliana]	Thylakoids plastoglobule
2321	Detail								leaf [A. thaliana]
2324	Detail								leaf [A. thaliana]	THYLAKOIDS thylakoid membrane

* For details about the exprimental sources click here.

Published Proteomics Data

18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
19995723(chloroplast reference proteome)
21515685(GreenCut2)

Comparative Proteomics Data