PPDB

The Plant Proteome Database

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AT2G39670.1   Radical SAM superfamily protein

Lab Annot. tRNA/rRNA RNA methyltransferase
Mapman: 27 RNA
Curated Location plastid (Pubmed: 23198870 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G39670.2 11Arabidopsis thaliana04319999115 (62)C
AT3G19630.1 11Arabidopsis thaliana9E-5828141601 (1)_
AT1G60230.1 11Arabidopsis thaliana3E-5434735520 (0)C
Os01g74190.1 7Oryza sativa035575885 (5)M
Os01g45370.1 7Oryza sativa1E-5438334500 (0)M
Os03g10520.1 7Oryza sativa7E-5127639560 (0)C
Zm00001eb362190_P003 agpv5Zea mays034875880 (0)M
GRMZM2G168412_P02 3.21Zea mays0348758829 (29)M
v1_GRMZM2G168412_P02 4a53Zea mays0348758822 (22)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Fer4_12(1) Fer4_14(1) Radical_SAM(2)
TargetP: Chloroplast (Class 4 C0.490; M0.271; S0.082; _0.022)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 428 aa (-cTP 378)
Molecular Weight 47.35 kDA(-cTP 41.99)
PI 9.05(-cTP 8.66)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
640Detail       leaf [A. thaliana] plastid nucleoid wild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1196Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1214Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1454Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 2)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1525Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1635Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1672Detail        [A. thaliana]  
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2321Detail       leaf [A. thaliana]  
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)

Comparative Proteomics Data


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