PPDB

 The Plant Proteome Database

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AT2G31810.1   ACT domain-containing small subunit of acetolactate synthase protein
Lab Annot. acetolactate synthase small subunit
Mapman: 13.1.4.1.1 amino acid metabolism.synthesis.branched chain group.common.acetolactate synthase
Curated Location plastid (Pubmed: 23198870 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G31810.3 11Arabidopsis thaliana04919595219 (114)C
AT2G31810.2 11Arabidopsis thaliana03879999133 (80)M
AT5G16290.2 11Arabidopsis thaliana0424708372 (39)C
Os02g39570.1 7Oryza sativa0411778728 (28)C
Os02g39570.4 7Oryza sativa0411778728 (28)C
Os11g14950.1 7Oryza sativa040872850 (0)C
GRMZM2G077215_P01 3.21Zea mays04107084100 (51)C
v1_GRMZM2G077215_P01 4a53Zea mays0410708428 (28)C
Zm00001eb112480_P005 agpv5Zea mays040771840 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: ACT(2) ACT_4(2) ACT_5(2) ALS_ss_C(3)
TargetP: Chloroplast (Class 2 C0.880; M0.128; S0.022; _0.028)
Predotar:
Subcel. Location: Lumen
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 491 aa (-cTP 464) (-lTP 373)
Molecular Weight 53.87 kDA(-cTP 51.19)(-lTP 41.41)
PI 9.07(-cTP 9.08)(-lTP 7.912)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
303Detail       leaf [A. thaliana] chloroplast stromawild-type
324Detail       leaf [A. thaliana] cell wall enriched wild-type
325Detail       BY2 cell suspension [Tobacco] cell wall enriched wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
541Detail       leaf [A. thaliana] stroma (FPLC) >1 MDa stromawild-type
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
1175Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1176Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1180Detail       leaf [A. thaliana] co-IP OEC23 stromaclpr2-1
1196Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1454Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 2)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1525Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1633Detail       leaf [A. thaliana] Stroma stromawild-type
1634Detail       leaf [A. thaliana] Stroma stromawild-type
1635Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1636Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1637Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1638Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1672Detail        [A. thaliana]  
1871Detail       Total leaf [A. thaliana] Stroma wild-type
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2050Detail       Total leaf [A. thaliana] total soluble wt and prep1-1xprep2-1xoop
2061Detail       Total leaf [A. thaliana] chloroplast stromawt and cgep
2064Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2065Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2068Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2069Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2072Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2073Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2074Detail       Total leaf [A. thaliana] total soluble wt & prep1xprep2xclpt1xclpt2*
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 17407188(cotyledon)
18431481(chloroplast)
19200160(flower-stage 12)
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
20423899(chloroplast)
19995723(chloroplast reference proteome)
21533090(plasmodesmata-crude)
21311031(chloroplasts)

Comparative Proteomics Data

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