PPDB

PPDB	The Plant Proteome Database

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AT2G31170.2 Cysteinyl-tRNA synthetase%2C class Ia family protein

Lab Annot.

Cys-tRNA synthetase class I (C) (dual targeted mito & plastid)
Mapman: 29.1.16 protein.aa activation.cysteine-tRNA ligase

Curated Location

mitochondria (Pubmed: 23198870 16251277 )
plastid stroma (Pubmed: 23198870 16251277 )

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT2G31170.3 11 Arabidopsis thaliana 0 401 100 100 0 (0) _
AT2G31170.1 11 Arabidopsis thaliana 0 401 100 100 124 (80) C
AT5G38830.1 11 Arabidopsis thaliana 2E-138 399 53 69 80 (46) _
Os09g38420.2 7 Oryza sativa 0 400 67 81 5 (5) _
Os09g38420.1 7 Oryza sativa 0 400 67 81 19 (19) M
Os03g04960.3 7 Oryza sativa 2E-164 417 55 70 3 (3) _
v1_GRMZM2G002851_P01 4a53 Zea mays 0 399 67 82 0 (0) _
Zm00001eb104530_P002 agpv5 Zea mays 0 399 67 82 0 (0) M
GRMZM2G002851_P01 3.21 Zea mays 0 399 67 82 0 (0) M
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	DALR_2(1) DUF2767(1) tRNA-synt_1(1) tRNA-synt_1e(1) tRNA-synt_1f(1) tRNA-synt_1g
TargetP:	Cytoplasm (Class 2 C0.086; M0.100; S0.154; _0.909)
Predotar:
Subcel. Location:
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	401 aa (-cTP 391)
Molecular Weight	45.29 kDA(-cTP )
PI	5.64(-cTP )

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
544	Detail								leaf [A. thaliana]	stroma (FPLC) <600 kDa (LMA)	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1399	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1401	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1403	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1407	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1410	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.2	wild-type
1411	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.1	clpr2-1
1412	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.2	clpr2-1
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1418	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 3)
1455	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1456	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1483	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1484	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 2)
1486	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 3)
1937	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1941	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1946	Detail								leaf [A. thaliana]	stroma UVR stroma	wild-type
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1948	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1987	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
2006	Detail								leaf [A. thaliana]	over expressed M48 plastoglobule	M48
2008	Detail								leaf [A. thaliana]	RNAi PG plastoglobule	RNAi
2009	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2010	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2020	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2113	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2115	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2117	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2400	Detail								leaf-soluble [A. thaliana]	GFP-nano-trap	wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
19995723(chloroplast reference proteome)
22060019(phosphoproteome - N-starved seedlings)
21841088(mitochondria (2DE native gel))

Comparative Proteomics Data