PPDB

PPDB	The Plant Proteome Database

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AT2G27450.1 nitrilase-like protein 1

Lab Annot.

NLP1 (NITRILASE-LIKE PROTEIN 1); hydrolase, acting on carbon-nitrogen bonds
Mapman: 22.1.5 polyamine metabolism.synthesis.N-carbamoylputrescine amidohydrolase

Curated Location

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT2G27450.2 11 Arabidopsis thaliana 0 326 91 91 30 (20) _
AT2G27450.3 11 Arabidopsis thaliana 4E-165 220 100 100 0 (0) S
AT5G64370.1 11 Arabidopsis thaliana 2E-21 274 30 47 39 (14) _
Os02g33080.1 7 Oryza sativa 2E-180 293 79 88 6 (6) _
Os02g33080.2 7 Oryza sativa 3E-140 219 84 90 6 (6) S
Os07g30170.1 7 Oryza sativa 2E-25 275 29 47 0 (0) _
v1_GRMZM2G073950_P01 4a53 Zea mays 2E-178 290 78 88 5 (5) S
Zm00001eb242790_P002 agpv5 Zea mays 2E-178 290 78 88 0 (0) S
GRMZM2G073950_P01 3.21 Zea mays 2E-178 290 78 88 3 (3) S
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	CN_hydrolase(1)
TargetP:	Cytoplasm (Class 3 C0.025; M0.293; S0.220; _0.750)
Predotar:
Subcel. Location:
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	299 aa (-cTP 264)
Molecular Weight	33.53 kDA(-cTP )
PI	5.71(-cTP )

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
361	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
371	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
451	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
527	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt1)
528	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt2)
1207	Detail								leaf [A. thaliana]	total leaf tissue	clpp3 null
1208	Detail								leaf [A. thaliana]	total leaf tissue	clpp3 null
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1530	Detail								leaf [A. thaliana]	total leaf tissue - 30 uM CAP	wild-type (rep. 1)
1531	Detail								leaf [A. thaliana]	total leaf tissue - 40 uM CAP	wild-type (rep. 1)
2013	Detail								leaf [A. thaliana]	total leaf tissue	wt
2014	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2015	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2018	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2324	Detail								leaf [A. thaliana]	THYLAKOIDS thylakoid membrane
2400	Detail								leaf-soluble [A. thaliana]	GFP-nano-trap	wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

19525416(leaf (wt and clpr4-1 mutant))
19888209(leaves)
19114538(guard cells Arabidopsis leaf)
21166475(cytosolic-fractions-crude)
21173025(root proteome)

Comparative Proteomics Data