PPDB

The Plant Proteome Database

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AT2G27020.1   20S proteasome alpha subunit G1

Lab Annot. 20S proteasome alpha subunit G (PAG1)
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: proteasome core complex (sensu Eukaryota)9373170,9611183
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G27020.2 11Arabidopsis thaliana02491001000 (0)_
AT3G14290.1 11Arabidopsis thaliana2E-372073355142 (87)_
AT1G53850.1 11Arabidopsis thaliana6E-372073255163 (105)_
Os01g59600.1 7Oryza sativa4E-163249849436 (36)_
Os01g59600.2 7Oryza sativa4E-163249849436 (36)_
Os05g41180.1 7Oryza sativa6E-16024984955 (5)_
v1_GRMZM2G445100_P02 4a53Zea mays1E-166249859513 (13)_
Zm00001eb150180_P001 agpv5Zea mays1E-16624985950 (0)_
Zm00001eb150180_P002 agpv5Zea mays1E-16624985950 (0)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Con-6(2) DUF4081(1) GA(2) Proteasome(1) Proteasome_A_N(1)
TargetP: Cytoplasm (Class 2 C0.068; M0.071; S0.180; _0.897)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 249 aa (-cTP 160)
Molecular Weight 27.38 kDA(-cTP )
PI 5.93(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
119Detail       root [B. rapa] plastid stroma 
120Detail       root [B. rapa] plastid stroma 
135Detail       leaf [A. thaliana] plastid thylakoid membranewild-type
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1583Detail       leaf [A. thaliana] TiO2 thylakoid membraneK2K3
1875Detail       Total leaf [A. thaliana] total soluble wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2077Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2086Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2161Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 31
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2219Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2223Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2266Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2303Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP

* For details about the exprimental sources click here.

Published Proteomics Data

15215502(Vacuolar proteome)
14617066(Nuclear proteome)
16358359(cell suspension (Gamborg))
16358359(cell suspension (M&Skoog))
16502469(cell suspension-Cd-up)
16055689(Glutathionylated suspension cell)
15815986(Leaf)
18538804(apoplast)
18538804(total leaf)
17407188(cotyledon)
17151019(vacuole - suspension cell)
19546170(mature pollen grains)
20118269(developing seeds)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
20405473(roots - TRX-y interacting proteins)
19334764(plasma membrane (cell culture))
19114538(guard cells Arabidopsis leaf)
19508356(mature pollen grains and pollen tubes)
20516081(tagged-26S proteasome-affinity)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data


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