PPDB

 The Plant Proteome Database

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AT2G21410.1   vacuolar proton ATPase A2
Lab Annot. VHA-A2 (VACUOLAR PROTON ATPASE A2); ATPase
Mapman: 34.1 transport.p- and v-ATPases
Curated Location vacuole-tonoplast (Pubmed: 16461582 )

TAIR curated location: mitochondrion & vacuolar membrane (sensu Magnoliophyta)14671022,16461582
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT4G39080.1 11Arabidopsis thaliana08118994325 (319)_
AT2G28520.1 11Arabidopsis thaliana080962791 (1)_
Os10g10500.1 7Oryza sativa0817758635 (35)_
Os03g14690.1 7Oryza sativa081074850 (0)_
Os01g61780.1 7Oryza sativa081160790 (0)_
Zm00001eb368270_P001 agpv5Zea mays062865820 (0)_
GRMZM2G104418_P02 3.21Zea mays039579890 (0)S
GRMZM2G104418_P01 3.21Zea mays039579890 (0)S
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: DivIC(2) DUF2730(2) DUF4200(1) V_ATPase_I(1)
TargetP: Cytoplasm (Class 2 C0.213; M0.129; S0.096; _0.866)
Predotar:
Subcel. Location:
TM-HMM prediction: 6 TMs
Aramemnon: 6 TMs
TAT position:
Length 821 aa (-cTP 757)
Molecular Weight 93.10 kDA(-cTP )
PI 5.38(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
1124Detail       leaf [A. thaliana] chloroplast plastoglobulewild-type
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1494Detail       leaf [A. thaliana]  
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1611Detail       leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1612Detail       Natural Senescence leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1614Detail       Natural Senescence leaf [A. thaliana]  
1617Detail        [A. thaliana] m48
1618Detail        [A. thaliana] m48
1627Detail       Rosettes [A. thaliana] total leaf tissue thylakoid + envelopeWild-type
1744Detail        [A. thaliana] Mep-6
1745Detail        [A. thaliana] Col-0
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
1829Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1 Fluctuating light
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2030Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2269Detail       seedlings [A. thaliana] total tissue wild-type
2320Detail        [A. thaliana] Thylakoids plastoglobule 

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
MitoDB(Mitochondrial proteome)
15215502(Vacuolar proteome)
15539469(vacuole)
16618929(vacuole-LOPIT-callus)
17151019(vacuole - suspension cell)
17432890(leaf total membranes)
17660356(tonoplast (Brassica oleracea buds))
19546170(mature pollen grains)
19036721(detergent resistant plasma membrane (DRM) proteins (Cell cultures))
19525416(leaf (wt and clpr4-1 mutant))
19334764(plasma membrane (cell culture))
20706207(contaminants cell cycle interactome (cell culture))
19114538(guard cells Arabidopsis leaf)
21826108(SYP trans-Golgi-filtered)
21533090(plasmodesmata-crude)
20843791(DRM-enriched plasmamembrane)
21173025(root proteome)
22430844(Golgi-cell-culture)
21988472(vacuole (unfiltered data))
21433285(Crude microsomes cell_cultures)
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data

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