PPDB

 The Plant Proteome Database

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AT2G20140.1   AAA-type ATPase family protein
Lab Annot. 26S protease regulatory complex subunit (RPT2b)
Mapman: 29.5.11.20.2* protein.degradation.ubiquitin.proteasome.26S
Curated Location cytosol
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT4G29040.1 11Arabidopsis thaliana04439999181 (43)_
AT5G58290.1 11Arabidopsis thaliana7E-1333625174366 (159)_
AT5G20000.1 11Arabidopsis thaliana3E-1123255272134 (42)_
Os07g49150.1 7Oryza sativa0448919525 (25)_
Os03g18690.1 7Oryza sativa0450919515 (15)_
Os02g21970.1 7Oryza sativa6E-131362507427 (27)C
Zm00001eb111910_P001 agpv5Zea mays043192960 (0)_
GRMZM2G095593_P01 3.21Zea mays0289969990 (35)_
GRMZM2G104373_P01 3.21Zea mays0447909536 (13)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: AAA(1) AAA_16(1) AAA_18(1) AAA_2(1) AAA_22(1) AAA_24(1) AAA_25(1) AAA_28(1) AAA_
TargetP: Cytoplasm (Class 2 C0.186; M0.043; S0.018; _0.905)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 443 aa (-cTP 396)
Molecular Weight 49.35 kDA(-cTP )
PI 5.85(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1018Detail        [aphid]  
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1449Detail       leaf [A. thaliana] total tissue (3 day HL) abck2xabck3 (rep. 2)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2267Detail       leaf [A. thaliana] stroma Clp Clp C1 TRAP
2272Detail       seedlings [A. thaliana] total tissue wild-type
2274Detail       seedlings [A. thaliana] total tissue wild-type
2289Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2298Detail        [A. thaliana] Clp C1 WT
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2304Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 17317660(Plasma Membrane proteome)
19200160(flower-stage 12)
19546170(mature pollen grains)
20118269(developing seeds)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19334764(plasma membrane (cell culture))
19888209(leaves)
20706207(cell cycle interactome (cell culture))
20516081(tagged-26S proteasome-affinity)
21325567(root-proteome (wt & det3))
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data

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