PPDB

 The Plant Proteome Database

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AT2G04350.1   AMP-dependent synthetase and ligase family protein
Lab Annot. long-chain acyl-CoA synthetase 8 (LACS8)
Mapman: 11.1.9 lipid metabolism.FA synthesis and FA elongation.long chain fatty acid CoA ligase
Curated Location ER (Pubmed: 21143684 )
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT2G04350.2 11Arabidopsis thaliana072010010032 (25)_
AT1G77590.1 11Arabidopsis thaliana06906680139 (123)C
AT1G77590.2 11Arabidopsis thaliana055070830 (0)S
Os05g25310.1 7Oryza sativa070368832 (2)_
Os05g25310.2 7Oryza sativa070368832 (2)_
Os12g07110.1 7Oryza sativa0694657844 (44)M
Zm00001eb195860_P003 agpv5Zea mays070367830 (0)_
v1_GRMZM2G339336_P01 4a53Zea mays072763781 (0)_
GRMZM2G118286_P01 3.21Zea mays0694637943 (43)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: AMP-binding(1) AMP-binding_C(1)
TargetP: Cytoplasm (Class 2 C0.074; M0.161; S0.099; _0.883)
Predotar:
Subcel. Location:
TM-HMM prediction: 2 TMs
Aramemnon: 1 TMs
TAT position:
Length 720 aa (-cTP 652)
Molecular Weight 78.34 kDA(-cTP )
PI 7.51(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1611Detail       leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1612Detail       Natural Senescence leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1613Detail       Natural Senescence leaf [A. thaliana]  
1614Detail       Natural Senescence leaf [A. thaliana]  
1615Detail       Natural Senescence leaf [A. thaliana]  
1616Detail       Natural Senescence leaf [A. thaliana]  
1621Detail        [A. thaliana] m48
1766Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light

* For details about the exprimental sources click here.

Published Proteomics Data

 16618929(ER-LOPIT-callus)
19546170(mature pollen grains)
19525416(leaf (wt and clpr4-1 mutant))
20061580(chloroplast envelope (inner+outer))
20843791(DRM-enriched plasmamembrane)
22430844(Golgi-cell-culture)
21988472(vacuole (unfiltered data))
21433285(Crude microsomes cell_cultures)
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data

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