PPDB

PPDB	The Plant Proteome Database

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AT1G80560.1 isopropylmalate dehydrogenase 2

Lab Annot.

3-isopropylmalate dehydrogenase-1
Mapman: 13.1.4.4.3 amino acid metabolism.synthesis.branched chain group.leucine specific.3-isopropylmalate dehydrogenase

Curated Location

plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT1G31180.1 11 Arabidopsis thaliana 0 403 85 92 283 (223) M
AT1G31180.2 11 Arabidopsis thaliana 0 403 85 92 0 (0) M
AT5G14200.1 11 Arabidopsis thaliana 0 409 84 91 335 (252) _
Os03g45320.1 7 Oryza sativa 0 370 81 90 36 (36) C
Os03g45320.2 7 Oryza sativa 1E-167 282 80 90 11 (11) C
Os01g16900.1 7 Oryza sativa 4E-41 339 31 50 24 (24) M
Zm00001eb076030_P001 agpv5 Zea mays 0 404 77 86 0 (0) M
GRMZM5G803490_P01 3.21 Zea mays 0 404 77 86 88 (85) M
v1_GRMZM2G145805_P01 4a53 Zea mays 0 404 76 86 71 (71) M
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	Iso_dh(1)
TargetP:	Chloroplast (Class 4 C0.645; M0.323; S0.045; _0.030)
Predotar:
Subcel. Location:	Stroma
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	405 aa (-cTP 373)
Molecular Weight	43.37 kDA(-cTP 39.86)
PI	5.80(-cTP 5.15)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
94	Detail								root [B. rapa]	plastid stroma
99	Detail								petal [B. oleracea]	plastid stroma
102	Detail								petal [B. oleracea]	plastid stroma	Alboglabra
119	Detail								root [B. rapa]	plastid stroma
151	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
180_181	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
326	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
339	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
351	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
352	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
355	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
361	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
371	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
451	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
527	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt1)
528	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt2)
544	Detail								leaf [A. thaliana]	stroma (FPLC) <600 kDa (LMA)	wild-type
618	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
621	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
622	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
873	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
874	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1127	Detail								leaf [A. thaliana]	chloroplast plastoglobule	k2k3
1392	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 1)
1395	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 1)
1399	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1400	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	wild-type
1401	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1403	Detail								leaf [A. thaliana]	total leaf tissue 1.14 -rep. 1	clpp3-1
1405	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	wild-type
1406	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	wild-type
1407	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1408	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1409	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.1	wild-type
1410	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.2	wild-type
1411	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.1	clpr2-1
1412	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.2	clpr2-1
1413	Detail								leaf [A. thaliana]	total leaf tissue -114 rep1tR1	wild-type
1416	Detail								leaf [A. thaliana]	total leaf tissue 1.14 rep 1.2	clpr2-1
1417	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 2)
1418	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 3)
1423	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (rep. 3)
1431	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	wild-type (rep. 2)
1432	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	wild-type (rep. 2)
1455	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1456	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1457	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1460	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 3)
1461	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 1)
1462	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 2)
1463	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 3)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1482	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 1)
1483	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1484	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 2)
1485	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1486	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 3)
1529	Detail								leaf [A. thaliana]	total leaf tissue - 0 uM CAP	wild-type (rep. 1)
1530	Detail								leaf [A. thaliana]	total leaf tissue - 30 uM CAP	wild-type (rep. 1)
1540	Detail								leaf [A. thaliana]	Thylakoids plastoglobule	wild-type
1541	Detail								leaf [A. thaliana]	Thylakoids plastoglobule	M-48
1826	Detail								leaf tip mesophyll [Zea Mays]	chloroplast stroma	W22 (repl. 3)
1872	Detail								Total leaf [A. thaliana]	Stroma	wild-type
1933	Detail								leaf [A. thaliana]	chloroplast	wild-type
1934	Detail								leaf [A. thaliana]	chloroplast	wild-type
1935	Detail								leaf [A. thaliana]	chloroplast	wild-type
1936	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1937	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1938	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1941	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1946	Detail								leaf [A. thaliana]	stroma UVR stroma	wild-type
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1948	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1949	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1983	Detail								leaf [A. thaliana]	plastid	wild-type
1984	Detail								leaf [A. thaliana]	plastid	wild-type
1985	Detail								leaf [A. thaliana]	plastid	wild-type
1986	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1987	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1988	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1999	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2004	Detail								leaf [A. thaliana]	WT PG plastoglobule	WT
2005	Detail								leaf [A. thaliana]	RNAi PG plastoglobule	RNAi
2009	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2010	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2011	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2012	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2013	Detail								leaf [A. thaliana]	total leaf tissue	wt
2014	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2015	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2016	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2017	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2018	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2019	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2020	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2113	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2115	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2117	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2182	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2183	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2184	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2185	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2193	Detail								[A. thaliana]	total soluble	P3-strepII 26
2216	Detail								leaf [A. thaliana]	total leaf tissue	Clp P5 st
2226	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2312	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2314	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2316	Detail								leaf [A. thaliana]	PG plastoglobule
2318	Detail								leaf [A. thaliana]	PG plastoglobule
2319	Detail								leaf [A. thaliana]	PG plastoglobule
2320	Detail								[A. thaliana]	Thylakoids plastoglobule
2321	Detail								leaf [A. thaliana]
2324	Detail								leaf [A. thaliana]	THYLAKOIDS thylakoid membrane
2398	Detail								leaf-soluble [A. thaliana]	GFP-nano-trap	wt/35SRecA-GFP
2400	Detail								leaf-soluble [A. thaliana]	GFP-nano-trap	wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

12938931(Total chloroplast envelope)
16207701(chloroplast stroma)
16358359(cell suspension (Gamborg))
15815986(seedling)
18431481(chloroplast)
18814325(cotyledons)
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
19888209(leaves)
19423572(leaf (wt and clpr2-1))
19995723(chloroplast reference proteome)
21173025(root proteome)

Comparative Proteomics Data