PPDB

The Plant Proteome Database

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AT1G74850.1   plastid transcriptionally active 2

Lab Annot. pentatricopeptide (PPR) repeat (pTAC2)
Mapman: 27.6* RNA.pentatricopeptide (PPR) repeat-containing protein
Curated Location plastid nucleoid (Pubmed: 23198870 16326926 18431481 ) (supported by in-house exprements)

TAIR curated location: plastid chromosome16326926
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G02860.1 11Arabidopsis thaliana3E-8259926500 (0)C
AT5G02860.5 11Arabidopsis thaliana3E-8259926500 (0)C
AT5G02860.3 11Arabidopsis thaliana3E-8259926500 (0)C
Os03g60910.1 7Oryza sativa0800547325 (25)_
Os05g19380.1 7Oryza sativa2E-75635254816 (16)C
Os07g40120.1 7Oryza sativa2E-7158125490 (0)C
Zm00001eb331750_P003 agpv5Zea mays079053730 (0)C
v1_GRMZM2G122116_P02 4a53Zea mays0791537349 (49)C
Zm00001eb331750_P002 agpv5Zea mays055252750 (0)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: Clathrin(3) ECSIT(5) Mtf2(5) PPR(16) PPR_1(14) PPR_2(15) PPR_3(10) PPR_long(5) R
TargetP: Chloroplast (Class 2 C0.821; M0.108; S0.021; _0.077)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 862 aa (-cTP 796)
Molecular Weight 96.32 kDA(-cTP 89.12)
PI 5.67(-cTP 5.23)

Experimental Evidence

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View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
303Detail       leaf [A. thaliana] chloroplast stromawild-type
327Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
343Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
542Detail       leaf [A. thaliana] stroma (FPLC) >500 kDa (HMB) wild-type
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
640Detail       leaf [A. thaliana] plastid nucleoid wild-type
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
875Detail       leaf [A. thaliana] chloroplast stromawild-type
876Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1175Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1176Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1178Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1196Detail       leaf [A. thaliana] co-IP RH3 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1214Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1215Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1454Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 2)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1525Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1633Detail       leaf [A. thaliana] Stroma stromawild-type
1634Detail       leaf [A. thaliana] Stroma stromawild-type
1635Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1636Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1637Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1638Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1639Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1670Detail        [A. thaliana]  
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1758Detail       leaf [A. thaliana] total leaf tissue DXS_Flag+fosmidomycin
1933Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2116Detail       leaf [A. thaliana] Stroma stromacGep
2117Detail       leaf [A. thaliana] Stroma stromacGep
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2251Detail       seedlings [A. thaliana] total tissue wild-type
2289Detail        [A. thaliana] Clp C1 TRAP
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

15028209(Total chloroplast)
16326926(nucleoids)
18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
20340159(chloroplast heparin-binding proteins)
19995723(chloroplast reference proteome)

Comparative Proteomics Data


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