PPDB

The Plant Proteome Database

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AT1G73650.1   3-oxo-5-alpha-steroid 4-dehydrogenase (DUF1295)

Lab Annot. oxidoreductase, acting on the CH-CH group of donors
Mapman: 35.2 not assigned.unknown
Curated Location plasma membrane
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   Uniprot   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G73650.5 11Arabidopsis thaliana7E-1532081001000 (0)S
AT1G73650.2 11Arabidopsis thaliana6E-152208999960 (58)S
AT1G73650.3 11Arabidopsis thaliana2E-15120610010059 (57)S
Os03g50070.1 7Oryza sativa2E-99206698215 (15)S
Os04g01510.2 7Oryza sativa2E-1418232490 (0)S
Os04g01510.1 7Oryza sativa2E-1418232490 (0)S
Zm00001eb216550_P005 agpv5Zea mays2E-10020067810 (0)S
Zm00001eb216550_P001 agpv5Zea mays3E-10020067810 (0)S
Zm00001eb216550_P003 agpv5Zea mays3E-10020067810 (0)S
Show all       

#Spots: The number of publicly accessible spots are in parenthesis

Prediction
PFAM: DUF1295(1) ICMT(1) PEMT(1) Steroid_dh(1)
TargetP: Cytoplasm (Class 5 C0.045; M0.719; S0.012; _0.737)
Predotar:
Subcel. Location:
TM-HMM prediction: 4 TMs
Aramemnon: 7 TMs
TAT position:
Length 208 aa (-cTP 164)
Molecular Weight 23.73 kDA(-cTP )
PI 8.99(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  

Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
1345Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 1)
1346Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 2)
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1348Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 1)
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1611Detail       leaf [A. thaliana] chloroplast fractionation thylakoid membrane 
1616Detail       Natural Senescence leaf [A. thaliana]  
1828Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0 Fluctuating light
2013Detail       leaf [A. thaliana] total leaf tissue wt
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2

* For details about the exprimental sources click here.

Published Proteomics Data

15060130(Plasma Membrane proteome)
15539469(vacuole)
17151019(vacuole - suspension cell)
17644812(plasma membrane - suspension cells)
19036721(detergent resistant plasma membrane (DRM) proteins (Cell cultures))
19525416(leaf (wt and clpr4-1 mutant))
19106119(plasma membrane detergent resistent microdomains (DRM))
19334764(plasma membrane (cell culture))
21533090(plasmodesmata-crude)
20843791(DRM-enriched plasmamembrane)
21173025(root proteome)
22430844(Golgi-cell-culture)
21988472(vacuole (unfiltered data))

Comparative Proteomics Data


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