PPDB

 The Plant Proteome Database

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AT1G69740.1   Aldolase superfamily protein
Lab Annot. porphobilinogen synthase-1 (delta-aminolevulinic acid dehydratase-1) (ALAD-1)
Mapman: 19.4 tetrapyrrole synthesis.ALA dehydratase
Curated Location plastid stroma (Pubmed: 23198870 3277625 18431481 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G69740.3 11Arabidopsis thaliana04301001000 (0)C
AT1G69740.2 11Arabidopsis thaliana0430100100578 (387)C
AT1G44318.1 11Arabidopsis thaliana042864770 (0)_
Os06g49110.3 7Oryza sativa0365838937 (37)C
Os06g49110.1 7Oryza sativa0365838937 (37)C
Os06g49110.2 7Oryza sativa4E-17231176842 (2)C
Zm00001eb227310_P001 agpv5Zea mays038879850 (0)C
v1_GRMZM2G397247_P04 4a53Zea mays03887985161 (161)C
GRMZM2G397247_P04 3.21Zea mays03887985189 (167)C
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: ALAD(1)
TargetP: Chloroplast (Class 2 C0.835; M0.060; S0.003; _0.208)
Predotar:
Subcel. Location: Lumen
TM-HMM prediction: No
Aramemnon:
TAT position: 46
Length 430 aa (-cTP 378) (-lTP 354)
Molecular Weight 46.69 kDA(-cTP 41.07)(-lTP 38.75)
PI 6.96(-cTP 5.46)(-lTP 5.606)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
94Detail       root [B. rapa] plastid stroma 
102Detail       petal [B. oleracea] plastid stromaAlboglabra
119Detail       root [B. rapa] plastid stroma 
151Detail       leaf [A. thaliana] chloroplast stromawild-type
180_181Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
187_188Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
195_194Detail       leaf [A. thaliana] chloroplast stromaffc1-2
249_250Detail       leaf [A. thaliana] chloroplast stromaffc1-2
272Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
303Detail       leaf [A. thaliana] chloroplast stromawild-type
326Detail       leaf [A. thaliana] chloroplast stromawild-type
327Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
343Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
355Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
420Detail       leaf [A. thaliana] chloroplast stromaclpR1-stop (EMS)
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
617Detail       leaf [A. thaliana] chloroplast stromawild-type
618Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
620Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
622Detail       leaf [A. thaliana] total leaf tissue wild-type
641Detail        [A. thaliana] plastid nucleoid (crude) wild-type
698Detail       leaf [A. thaliana] stroma- QconCAT wild-type
869Detail       leaf [A. thaliana] chloroplast stromawild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
873Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
875Detail       leaf [A. thaliana] chloroplast stromawild-type
876Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1113Detail        [A. thaliana] control for RARE-1 co-IP wild-type
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1208Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1331Detail       leaf [A. thaliana] chloroplast thylakoid membranewild-type
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1413Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR1 wild-type
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1416Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.2 clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1421Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1422Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1423Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1424Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 1)
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1426Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 3)
1431Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1439Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 1)
1440Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1442Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 1)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1445Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 1)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1447Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 3)
1450Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1456Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1459Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 2)
1460Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 3)
1461Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1463Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 3)
1465Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 1)
1467Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 2)
1469Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 3)
1471Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clps (rep. 1-2)
1475Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 4)
1477Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 5)
1479Detail       leaf [A. thaliana] co-IP GST-ClpS stroma clpc1 x clps (rep. 6)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1482Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 1)
1483Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 2)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1526Detail       leaf [A. thaliana] co-IP GST-ClpS 100ug bait stromaclpc1 x clps (rep. 2)
1527Detail       leaf [A. thaliana] co-IP GST-ClpS 10ug bait stromaclpc1 x clps (rep. 2)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1533Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
1597Detail        [A. thaliana]  
1700Detail        [A. thaliana] Col-0
1701Detail        [A. thaliana] cGep
1703Detail        [A. thaliana] cGep
1724Detail        [A. thaliana]  
1826Detail       leaf tip mesophyll [Zea Mays] chloroplast stromaW22 (repl. 3)
1870Detail       Total leaf [A. thaliana] Stroma wild-type
1871Detail       Total leaf [A. thaliana] Stroma wild-type
1872Detail       Total leaf [A. thaliana] Stroma wild-type
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1876Detail       Total leaf [A. thaliana] total soluble wild-type
1930Detail       leaf [A. thaliana] total leaf tissue Col_0 Wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1932Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1933Detail       leaf [A. thaliana] chloroplast wild-type
1934Detail       leaf [A. thaliana] chloroplast wild-type
1935Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2010Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2076Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2083Detail       Total leaf [A. thaliana] total soluble wt & clpt1xclpt2
2087Detail       Total leaf [A. thaliana] total soluble wild-type
2094Detail       leaf chloroplasts [A. thaliana] stroma stroma 
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2116Detail       leaf [A. thaliana] Stroma stromacGep
2132Detail       leaf [A. thaliana] chloroplast fractionation MSH1
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2161Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 31
2167Detail       leaf [A. thaliana] stroma Clp stromaP3 trap-strepII
2168Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII
2179Detail       leaf [A. thaliana] stroma Clp GST ClpF x ClpS1
2180Detail       leaf [A. thaliana] stroma Clp ClpF
2181Detail       leaf [A. thaliana] stroma Clp ClpF x ClpS1
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2190Detail        [A. thaliana] plastid stroma stromaP3-strepII 31
2191Detail        [A. thaliana] plastid stroma stromaP3-trap-strepII 40
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2193Detail        [A. thaliana] total soluble P3-strepII 26
2209Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 10
2211Detail       leaf [A. thaliana] total leaf tissue Clp P5 Trapst 12
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2224Detail       leaf [A. thaliana] total leaf tissue Clp P3 strep
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2245Detail       seedlings [A. thaliana] total tissue wild-type
2246Detail       seedlings [A. thaliana] total tissue gfs9
2247Detail       seedlings [A. thaliana] total tissue atg5
2248Detail       seedlings [A. thaliana] total tissue wild-type
2249Detail       seedlings [A. thaliana] total tissue gfs9
2250Detail       seedlings [A. thaliana] total tissue atg5
2251Detail       seedlings [A. thaliana] total tissue wild-type
2252Detail       seedlings [A. thaliana] total tissue gfs9
2253Detail       seedlings [A. thaliana] total tissue atg5
2262Detail       leaf [A. thaliana] stroma Clp Clp C1 wild-type
2271Detail       seedlings [A. thaliana] total tissue wild-type
2285Detail        [A. thaliana] Clp C1 TRAP
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2298Detail        [A. thaliana] Clp C1 WT
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 15028209(Total chloroplast)
16207701(chloroplast stroma)
18633119(stroma)
18431481(chloroplast)
19546170(mature pollen grains)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
19525416(leaf (wt and clpr4-1 mutant))
17216043(leaf)
20423899(chloroplast)
19888209(80S polysomal fraction)
19888209(leaves)
19423572(leaf (wt and clpr2-1))
19423572(stroma chloroplast (wt and clpr2-1))
20061580(chloroplast envelope (inner+outer))
19995723(chloroplast reference proteome)
21311031(chloroplasts)
21173025(root proteome)
21472856(mitochondrial-2DEgels-uncurated)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
180_181clpr2-1 cICAT - exp1 / wt cICAT - exp1cICAT5EDGVIMNDETVHQLCK + ICAT_l / EDGVIMNDETVHQLCK + ICAT_h22.22 leaf [A. thaliana] chloroplast stroma
180_181clpr2-1 cICAT - exp1 / wt cICAT - exp1cICAT5VMMESLMCLR + Oxid (M); ICAT_l / VMMESLMCLR + Oxid (M); ICAT_h22.04 leaf [A. thaliana] chloroplast stroma
195_194ffc1-2 cICAT- exp1 / wt cICAT- exp1cICAT10R.EDGVIMNDETVHQLCK.Q + ICAT_l / R.EDGVIMNDETVHQLCK.Q + ICAT_h31.02 leaf [A. thaliana] chloroplast stroma

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