PPDB

 The Plant Proteome Database

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AT1G56050.1   GTP-binding protein-like protein
Lab Annot. GTPase EngD-2 (ENGD-2)
Mapman: 30.5 signalling.G-proteins
Curated Location plastid stroma (Pubmed: 23198870 18431481 25566271 ) (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G30580.1 11Arabidopsis thaliana3E-903874058314 (128)_
AT5G18570.1 11Arabidopsis thaliana1E-10100365591 (45)C
AT1G07615.1 11Arabidopsis thaliana2E-1010037491 (1)M
Os03g56840.1 7Oryza sativa0419728426 (26)C
Os03g56840.2 7Oryza sativa0399708426 (26)C
Os08g09940.1 7Oryza sativa7E-88381385829 (29)_
GRMZM2G038494_P01 3.21Zea mays03847690159 (70)M
Zm00001eb059620_P002 agpv5Zea mays038476906 (6)M
v1_GRMZM2G038494_P03 4a53Zea mays0384769052 (52)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: FeoB_N(2) MMR_HSR1(1) YchF-GTPase_C(1)
TargetP: Chloroplast (Class 5 C0.304; M0.085; S0.157; _0.003)
Predotar:
Subcel. Location: Stroma
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 421 aa (-cTP 348)
Molecular Weight 45.63 kDA(-cTP 38.00)
PI 5.73(-cTP 5.02)

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
151Detail       leaf [A. thaliana] chloroplast stromawild-type
180_181Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
187_188Detail       leaf [A. thaliana] chloroplast stromaclpr2-1
249_250Detail       leaf [A. thaliana] chloroplast stromaffc1-2
303Detail       leaf [A. thaliana] chloroplast stromawild-type
339Detail       leaf [A. thaliana] chloroplast stromawild-type
342Detail       leaf [A. thaliana] chloroplast low density membranewild-type
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
356Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
371Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
543Detail       leaf [A. thaliana] stroma (FPLC) >600 kDa (HMA) wild-type
544Detail       leaf [A. thaliana] stroma (FPLC) <600 kDa (LMA) wild-type
870Detail       leaf [A. thaliana] chloroplast stromawild-type
871Detail       leaf [A. thaliana] chloroplast stromawild-type
874Detail       leaf [A. thaliana] chloroplast stromawild-type
877Detail       leaf [A. thaliana] chloroplast stromawild-type
1176Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1179Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1197Detail       leaf [A. thaliana] co-IP ClpR2 stromaclpr2-1
1214Detail       leaf [A. thaliana] co-IP RH3 stromawild-type
1215Detail       leaf [A. thaliana] co-IP ClpR2 stromawild-type
1291Detail       leaf [A. thaliana] co-IP preserum cGEP (rep. 1) stromawild-type
1292Detail       leaf [A. thaliana] co-IP cGEP (rep. 1) stromawild-type
1293Detail       leaf [A. thaliana] co-IP preserum cGEP (rep. 2) stromawild-type
1294Detail       leaf [A. thaliana] co-IP cGEP (rep. 2) stromawild-type
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1399Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1411Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 clpr2-1
1412Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 clpr2-1
1414Detail       leaf [A. thaliana] total leaf tissue -114 rep1tR2 wild-type
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1455Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1457Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1458Detail       leaf [A. thaliana] chloroplast stromaclps (rep. 1)
1462Detail       leaf [A. thaliana] chloroplast stromaclpc1 (rep. 2)
1481Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 1)
1484Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 2)
1485Detail       leaf [A. thaliana] chloroplast stromawild-type (rep. 3)
1486Detail       leaf [A. thaliana] chloroplast stromaclpc1 x clps (rep. 3)
1530Detail       leaf [A. thaliana] total leaf tissue - 30 uM CAP wild-type (rep. 1)
1569Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1572Detail       leaf [A. thaliana] TiO2 thylakoid membraneWT
1596Detail        [A. thaliana]  
1634Detail       leaf [A. thaliana] Stroma stromawild-type
1636Detail       leaf [A. thaliana] Stroma stromaclpt1-2
1637Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1638Detail       leaf [A. thaliana] total leaf tissue stromaWild-type
1640Detail       leaf [A. thaliana] total leaf tissue stromaclpt2-1
1934Detail       leaf [A. thaliana] chloroplast wild-type
1936Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1937Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1938Detail       leaf [A. thaliana] chloroplast Prep1_Prep2
1941Detail       leaf [A. thaliana] stroma WT stromawild-type
1942Detail       leaf [A. thaliana] stroma WT stromawild-type
1943Detail       leaf [A. thaliana] stroma WT stromawild-type
1944Detail       leaf [A. thaliana] stroma UVR stromaUVR
1945Detail       leaf [A. thaliana] stroma UVR stromaUVR
1946Detail       leaf [A. thaliana] stroma UVR stromawild-type
1947Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1948Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1949Detail       leaf [A. thaliana] stroma ClpS stromaclpS
1983Detail       leaf [A. thaliana] plastid wild-type
1984Detail       leaf [A. thaliana] plastid wild-type
1985Detail       leaf [A. thaliana] plastid wild-type
1986Detail       leaf [A. thaliana] plastid Prep1_Prep2
1987Detail       leaf [A. thaliana] plastid Prep1_Prep2
1988Detail       leaf [A. thaliana] plastid Prep1_Prep2
2009Detail       leaf [A. thaliana] total leaf tissue wild-type
2012Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2014Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2015Detail       leaf [A. thaliana] total leaf tissue opda1-2
2016Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2017Detail       leaf [A. thaliana] total leaf tissue wild-type
2018Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2112Detail       leaf [A. thaliana] Stroma stromawt
2113Detail       leaf [A. thaliana] Stroma stromawt
2114Detail       leaf [A. thaliana] Stroma stromawt
2115Detail       leaf [A. thaliana] Stroma stromacGep
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2183Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2247Detail       seedlings [A. thaliana] total tissue atg5
2286Detail        [A. thaliana] Clp C1 WT
2287Detail        [A. thaliana] Clp C1 WT
2288Detail        [A. thaliana] Clp C1 WT
2289Detail        [A. thaliana] Clp C1 TRAP
2290Detail        [A. thaliana] Clp C1 TRAP
2291Detail        [A. thaliana] Clp C1 TRAP
2296Detail        [A. thaliana] Clp C1 WT
2297Detail        [A. thaliana] Clp C1 WT
2299Detail        [A. thaliana] Clp C1 TRAP
2300Detail        [A. thaliana] Clp C1 TRAP
2301Detail        [A. thaliana] Clp C1 TRAP
2302Detail        [A. thaliana] Clp C1 WT
2303Detail        [A. thaliana] Clp C1 WT
2305Detail        [A. thaliana] Clp C1 TRAP
2306Detail        [A. thaliana] Clp C1 TRAP
2307Detail        [A. thaliana] Clp C1 TRAP
2310Detail       leaf [A. thaliana] PG plastoglobule 
2311Detail       leaf [A. thaliana] PG plastoglobule 
2312Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2313Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2314Detail       leaf [A. thaliana] thylakoids thylakoid membrane 
2316Detail       leaf [A. thaliana] PG plastoglobule 
2317Detail       leaf [A. thaliana] PG plastoglobule 
2318Detail       leaf [A. thaliana] PG plastoglobule 
2319Detail       leaf [A. thaliana] PG plastoglobule 
2320Detail        [A. thaliana] Thylakoids plastoglobule 
2321Detail       leaf [A. thaliana]  
2324Detail       leaf [A. thaliana] THYLAKOIDS thylakoid membrane 
2325Detail       leaf [A. thaliana] thylakoids thylakoid membraneabc1k6
2400Detail       leaf-soluble [A. thaliana] GFP-nano-trap wt/DUF760-1GFP/ClpC1TRAPSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

 16207701(chloroplast stroma)
18633119(stroma)
18431481(chloroplast)
17216043(leaf)
20423899(chloroplast)
19888209(leaves)
19423572(stroma chloroplast (wt and clpr2-1))
19995723(chloroplast reference proteome)
21173025(root proteome)

Comparative Proteomics Data
Expr*SourceTech.SpotPepSeqChargeState(z)AreaRatioAmbiguityTissueSample from
180_181clpr2-1 cICAT - exp1 / wt cICAT - exp1cICAT5SDIDVINLELIFCDLDQIGK + ICAT_l / SDIDVINLELIFCDLDQIGK + ICAT_h20.97 leaf [A. thaliana] chloroplast stroma

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