PPDB

 The Plant Proteome Database

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AT1G54270.2   eif4a-2
Lab Annot. initiation factor 4A-2 (eIF-4A-2), DEAD box RNA helicase (RH19)
Mapman: 29.2.3 protein.synthesis.initiation; 27.5* RNA.DEAD BOX helicase
Curated Location not plastid (supported by in-house exprements)
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links PeptideAtlas   POGS   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT1G54270.1 11Arabidopsis thaliana04129898946 (446)_
AT3G13920.4 11Arabidopsis thaliana04079698203 (134)_
AT3G13920.1 11Arabidopsis thaliana041295971139 (622)_
Os06g48750.1 7Oryza sativa0414909579 (79)_
Os06g48750.2 7Oryza sativa0414909578 (78)_
Os02g05330.1 7Oryza sativa0414909430 (30)_
GRMZM2G027995_P01 3.21Zea mays04148994314 (204)_
GRMZM2G027995_P02 3.21Zea mays04148994314 (204)_
Zm00001eb272920_P004 agpv5Zea mays041489942 (2)_
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: AAA_19(2) AAA_22(1) AAA_30(1) CMS1(2) DEAD(1) Flavi_DEAD(1) Helicase_C(3) ResIII
TargetP: Cytoplasm (Class 2 C0.066; M0.155; S0.146; _0.899)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 407 aa (-cTP 405)
Molecular Weight 46.19 kDA(-cTP )
PI 5.45(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
1347Detail       leaf [A. thaliana] total tissue (5 day HL) wild-type (rep. 3)
1350Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 3)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1402Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1404Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1405Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1407Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1408Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. clpr2-1
1417Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 2)
1418Detail       leaf [A. thaliana] total tissue (2% sucrose) clpr4-1 (rep. 3)
1432Detail       leaf [A. thaliana] total tissue (2% sucrose) wild-type (rep. 2)
1441Detail       leaf [A. thaliana] total tissue (0 day HL) wild-type (rep. 3)
1443Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 2)
1444Detail       leaf [A. thaliana] total tissue (0 day HL) abck2xabck3 (rep. 3)
1446Detail       leaf [A. thaliana] total tissue (3 day HL) wild-type (rep. 2)
1453Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1528Detail       leaf [A. thaliana] chloroplast nucleoidwild-type (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1535Detail       leaf [A. thaliana] wild-type
1547Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1548Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1551Detail       leaf [A. thaliana] TiO2 thylakoid membranek1k3
1552Detail       leaf [A. thaliana] TiO2 thylakoid membranewt
1618Detail        [A. thaliana] m48
1620Detail        [A. thaliana] m48
1621Detail        [A. thaliana] m48
1709Detail       Rosettes [A. thaliana] Thylakoids thylakoid membraneWild-type
1756Detail       leaf [A. thaliana] total leaf tissue Col_0
1757Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
1765Detail       leaf [A. thaliana] Thylakoids thylakoid membraneCol-0
1833Detail       leaf [A. thaliana] Thylakoids thylakoid membraneMet1-1
1875Detail       Total leaf [A. thaliana] total soluble wild-type
1926Detail       leaf [A. thaliana] total leaf tissue k1xk3 mutant (TiO2)
1928Detail       Rosettes [A. thaliana] Thylakoids wild-type
1929Detail       Rosettes [A. thaliana] Thylakoids wild-type
1931Detail       leaf [A. thaliana] total leaf tissue DXS_Flag
2005Detail       leaf [A. thaliana] RNAi PG plastoglobuleRNAi
2006Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2029Detail       leaf [A. thaliana] over expressed M48 plastoglobuleM48
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2222Detail       Total soluble [A. thaliana] Total soluble proteins cGEP strep
2296Detail        [A. thaliana] Clp C1 WT

* For details about the exprimental sources click here.

Published Proteomics Data

 21826108(SYP trans-Golgi-filtered)
21533090(plasmodesmata-crude)
21166475(cytosolic-fractions-crude)
20733066(phosphoproteome-ABA-leaf)
21173025(root proteome)
22430844(Golgi-cell-culture)
21988472(vacuole (unfiltered data))
21189294(nuclear pore complex (co-IPs RAE1,Nup43,50a,93a,136))
21433285(purified Plasma membrane -cell_cultures)

Comparative Proteomics Data

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