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AT1G49630.1 presequence protease 2

Lab Annot.

PREP2 - metalloprotease dually targeted mito & plastid
Mapman: 29.5.7.4.1* protein.degradation.metalloprotease.ME.M16

Curated Location

mitochondria (Pubmed: 23198870 15827031 )
plastid stroma (Pubmed: 23198870 15827031 )

TAIR curated location: mitochondrion & chloroplast15827031

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT1G49630.2 11 Arabidopsis thaliana 0 1080 100 100 95 (45) C
AT1G49630.3 11 Arabidopsis thaliana 0 1080 100 100 95 (45) C
AT1G49630.5 11 Arabidopsis thaliana 0 927 100 100 0 (0) _
Os02g52390.1 7 Oryza sativa 0 1004 75 88 74 (74) M
Zm00001eb255690_P001 agpv5 Zea mays 0 1042 74 87 12 (12) M
v1_GRMZM2G101271_P01 4a53 Zea mays 0 489 72 87 119 (119) _
GRMZM2G101271_P01 3.21 Zea mays 0 489 72 87 159 (118) _
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	DUF2119(1) M16C_assoc(1) Peptidase_M16(1) Peptidase_M16_C(2)
TargetP:	Chloroplast (Class 3 C0.870; M0.353; S0.005; _0.007)
Predotar:
Subcel. Location:	Stroma
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	1080 aa (-cTP 1012)
Molecular Weight	121.13 kDA(-cTP 113.58)
PI	5.33(-cTP 5.05)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
94	Detail								root [B. rapa]	plastid stroma
119	Detail								root [B. rapa]	plastid stroma
127	Detail								leaf [A. thaliana]	plastid thylakoid membrane	wild-type
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
361	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
371	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
527	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt1)
528	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt2)
869	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
871	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1405	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	wild-type
1407	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1408	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1411	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 1.1	clpr2-1
1461	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 1)
1479	Detail								leaf [A. thaliana]	co-IP GST-ClpS stroma	clpc1 x clps (rep. 6)
1486	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 3)
1826	Detail								leaf tip mesophyll [Zea Mays]	chloroplast stroma	W22 (repl. 3)
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1946	Detail								leaf [A. thaliana]	stroma UVR stroma	wild-type
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1949	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
2001	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2002	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2113	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2182	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2183	Detail								Rosettes [A. thaliana]	total soluble	tic40 and wt
2193	Detail								[A. thaliana]	total soluble	P3-strepII 26
2314	Detail								leaf [A. thaliana]	thylakoids thylakoid membrane
2316	Detail								leaf [A. thaliana]	PG plastoglobule
2399	Detail								leaf-soluble [A. thaliana]	GFP-nano-trap	wt/DUF760-1GFP/ClpC1WTSTREP

* For details about the exprimental sources click here.

Published Proteomics Data

18431481(chloroplast)
19423572(leaf (wt and clpr2-1))
19995723(chloroplast reference proteome)
21166475(cytosolic-fractions-crude)
21173025(root proteome)
21433285(Crude microsomes cell_cultures)

Comparative Proteomics Data