PPDB

 The Plant Proteome Database

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AT1G47250.1   20S proteasome alpha subunit F2
Lab Annot. 20S proteasome alpha subunit F2 (PAF2) (PRC2B) (PRS1
Mapman: 29.5.11.20.1* protein.degradation.ubiquitin.proteasome.20S
Curated Location cytosol

TAIR curated location: proteasome core complex (sensu Eukaryota)9373170,9611183
Species Arabidopsis thaliana     Source: TAIR Arabidopsis (v.11)
Links TAIR   PeptideAtlas   POGS   SUBA   Uniprot   PTM     Get sequence
Related Genes

AccverSpeciesEvalueMatchlen%Iden%Similar#spotsTargetP
AT5G42790.1 11Arabidopsis thaliana9E-1792788993261 (109)_
AT3G14290.1 11Arabidopsis thaliana4E-452433853171 (84)_
AT1G53850.1 11Arabidopsis thaliana5E-452373757194 (103)_
Os02g04100.1 7Oryza sativa2E-129240748536 (36)_
Os11g40140.1 7Oryza sativa5E-44237365769 (69)_
Os06g07140.1 7Oryza sativa5E-38254335419 (19)M
Zm00001eb231490_P001 agpv5Zea mays1E-13124073840 (0)_
GRMZM6G869379_P02 3.21Zea mays1E-131240738479 (65)_
GRMZM6G869379_P01 3.21Zea mays3E-102203698124 (15)M
Show all       

#Spots: The number of publicly accessible spots are in parenthesis
Prediction
PFAM: Dpoe2NT(1) Proteasome(1) Proteasome_A_N(1)
TargetP: Cytoplasm (Class 3 C0.033; M0.453; S0.009; _0.866)
Predotar:
Subcel. Location:
TM-HMM prediction: No
Aramemnon:
TAT position:
Length 277 aa (-cTP 253)
Molecular Weight 30.41 kDA(-cTP )
PI 4.97(-cTP )

Experimental Evidence

Expand

View Identified Peptides  

View GeneModel  
Expr*SpotMW nativeMW denaturedpI nativepI denaturedTypeMowseAmbiguityTissueSample fromGenotype
310Detail       leaf [A. thaliana] total leaf tissue clpr2-1:R2-TAPlong
351Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
352Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
361Detail       leaf [A. thaliana] total leaf tissue 1.07 wild-type
451Detail       leaf [A. thaliana] total leaf tissue clpr4-1
527Detail       leaf [A. thaliana] total leaf tissue wild-type (wt1)
528Detail       leaf [A. thaliana] total leaf tissue wild-type (wt2)
621Detail       leaf [A. thaliana] total leaf tissue clpr4-1
1202Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1207Detail       leaf [A. thaliana] total leaf tissue clpp3 null
1349Detail       leaf [A. thaliana] total tissue (5 day HL) abck2xabck3 (rep. 2)
1392Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1393Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 2)
1394Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1395Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 1)
1396Detail       leaf [A. thaliana] total leaf tissue clpt1xclpt2 (rep. 2)
1397Detail       leaf [A. thaliana] total leaf tissue wild-type (rep. 3)
1400Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 wild-type
1401Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1403Detail       leaf [A. thaliana] total leaf tissue 1.14 -rep. 1 clpp3-1
1406Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 2. wild-type
1409Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.1 wild-type
1410Detail       leaf [A. thaliana] total leaf tissue 1.07 rep 1.2 wild-type
1415Detail       leaf [A. thaliana] total leaf tissue 1.14 rep 1.1 clpr2-1
1425Detail       leaf [A. thaliana] total leaf tissue prep1xprep2xt1xt2 (rep. 2)
1529Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP wild-type (rep. 1)
1531Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP wild-type (rep. 1)
1532Detail       leaf [A. thaliana] total leaf tissue - 0 uM CAP clps (rep. 1)
1534Detail       leaf [A. thaliana] total leaf tissue - 40 uM CAP clps (rep. 1)
1596Detail        [A. thaliana]  
2011Detail       leaf [A. thaliana] total leaf tissue opda1-2
2013Detail       leaf [A. thaliana] total leaf tissue wt
2019Detail       leaf [A. thaliana] total leaf tissue opda1-2
2020Detail       leaf [A. thaliana] total leaf tissue prep1-1xprep2-1xopda1-2
2160Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 13
2161Detail       leaf [A. thaliana] stroma Clp stromaP3-strepII 31
2182Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2184Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2185Detail       Rosettes [A. thaliana] total soluble tic40 and wt
2192Detail        [A. thaliana] total soluble P3-trap-strepII 16
2208Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII
2210Detail       leaf [A. thaliana] total leaf tissue Clp P5 strepII st28
2214Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2215Detail       leaf [A. thaliana] total leaf tissue Clp P5 trap st
2216Detail       leaf [A. thaliana] total leaf tissue Clp P5 st
2225Detail       leaf [A. thaliana] total leaf tissue Clp P3 trap
2226Detail       leaf [A. thaliana] total leaf tissue wild-type
2248Detail       seedlings [A. thaliana] total tissue wild-type
2251Detail       seedlings [A. thaliana] total tissue wild-type

* For details about the exprimental sources click here.

Published Proteomics Data

 17317660(Plasma Membrane proteome)
19429840(ubiquitinated or associated with ubiquitinated proteins (seedlings))
20516081(tagged-26S proteasome-affinity)
21533090(plasmodesmata-crude)
21166475(cytosolic-fractions-crude)
21166475(cytosol-enriched&curated)
21173025(root proteome)

Comparative Proteomics Data

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