PPDB

PPDB	The Plant Proteome Database

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AT1G35420.1 alpha/beta-Hydrolases superfamily protein

Lab Annot.

dienelactone hydrolase family
Mapman: 26.57* misc.alpha/beta-Hydrolase family

Curated Location

plastid stroma (Pubmed: 23198870 18431481 ) (supported by in-house exprements)

Species

Arabidopsis thaliana Source: TAIR Arabidopsis (v.11)

Links

TAIR PeptideAtlas POGS SUBA Uniprot PTM Get sequence

Related Genes

Acc ver Species Evalue Matchlen %Iden %Similar #spots TargetP
AT1G35420.2 11 Arabidopsis thaliana 0 315 98 98 80 (49) C
AT1G35420.3 11 Arabidopsis thaliana 0 274 98 98 0 (0) M
AT3G23600.1 11 Arabidopsis thaliana 2E-13 201 27 47 320 (191) _
Os05g35470.1 7 Oryza sativa 1E-91 250 56 72 4 (4) C
Os08g14109.1 7 Oryza sativa 1E-21 225 29 47 0 (0) S
Os08g14000.1 7 Oryza sativa 4E-21 212 29 48 1 (1) S
GRMZM2G042516_P01 3.21 Zea mays 2E-101 287 52 66 21 (15) C
v1_GRMZM2G042516_P01 4a53 Zea mays 2E-101 287 52 66 20 (20) C
Zm00001eb348780_P003 agpv5 Zea mays 2E-101 287 52 66 2 (2) C
Show all

#Spots: The number of publicly accessible spots are in parenthesis

Prediction

PFAM:	Abhydrolase_1(2) Abhydrolase_4(1) DLH(1) EBV-NA3(1) Hydrolase_4(2) Peptidase_M55
TargetP:	Chloroplast (Class 3 C0.882; M0.365; S0.016; _0.006)
Predotar:
Subcel. Location:	Stroma
TM-HMM prediction:	No
Aramemnon:
TAT position:
Length	310 aa (-cTP 273)
Molecular Weight	34.17 kDA(-cTP 30.32)
PI	5.27(-cTP 4.88)

Experimental Evidence

Expand

View Identified Peptides

View GeneModel

Expr*	Spot	MW native	MW denatured	pI native	pI denatured	Type	Mowse	Ambiguity	Tissue	Sample from	Genotype
151	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
187_188	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
303	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
326	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
361	Detail								leaf [A. thaliana]	total leaf tissue 1.07	wild-type
371	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
451	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
527	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt1)
528	Detail								leaf [A. thaliana]	total leaf tissue	wild-type (wt2)
544	Detail								leaf [A. thaliana]	stroma (FPLC) <600 kDa (LMA)	wild-type
618	Detail								leaf [A. thaliana]	chloroplast stroma	clpr2-1
621	Detail								leaf [A. thaliana]	total leaf tissue	clpr4-1
622	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
869	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
870	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
873	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
874	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type
1347	Detail								leaf [A. thaliana]	total tissue (5 day HL)	wild-type (rep. 3)
1407	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1408	Detail								leaf [A. thaliana]	total leaf tissue 1.07 rep 2.	clpr2-1
1418	Detail								leaf [A. thaliana]	total tissue (2% sucrose)	clpr4-1 (rep. 3)
1440	Detail								leaf [A. thaliana]	total tissue (0 day HL)	wild-type (rep. 2)
1442	Detail								leaf [A. thaliana]	total tissue (0 day HL)	abck2xabck3 (rep. 1)
1455	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1456	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 2)
1457	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1458	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 1)
1459	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 2)
1460	Detail								leaf [A. thaliana]	chloroplast stroma	clps (rep. 3)
1461	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 1)
1462	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 2)
1463	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 (rep. 3)
1481	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 1)
1482	Detail								leaf [A. thaliana]	chloroplast stroma	clpc1 x clps (rep. 1)
1485	Detail								leaf [A. thaliana]	chloroplast stroma	wild-type (rep. 3)
1936	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1937	Detail								leaf [A. thaliana]	chloroplast	Prep1_Prep2
1941	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1942	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1943	Detail								leaf [A. thaliana]	stroma WT stroma	wild-type
1944	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1945	Detail								leaf [A. thaliana]	stroma UVR stroma	UVR
1946	Detail								leaf [A. thaliana]	stroma UVR stroma	wild-type
1947	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1948	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS
1988	Detail								leaf [A. thaliana]	plastid	Prep1_Prep2
1999	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2000	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2001	Detail								leaf [A. thaliana]	stroma ClpS stroma	clpS1
2009	Detail								leaf [A. thaliana]	total leaf tissue	wild-type
2010	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2011	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2012	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2014	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1
2015	Detail								leaf [A. thaliana]	total leaf tissue	opda1-2
2016	Detail								leaf [A. thaliana]	total leaf tissue	prep1-1xprep2-1xopda1-2
2112	Detail								leaf [A. thaliana]	Stroma stroma	wt
2113	Detail								leaf [A. thaliana]	Stroma stroma	wt
2114	Detail								leaf [A. thaliana]	Stroma stroma	wt
2115	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2116	Detail								leaf [A. thaliana]	Stroma stroma	cGep
2117	Detail								leaf [A. thaliana]	Stroma stroma	cGep

* For details about the exprimental sources click here.

Published Proteomics Data

16207701(chloroplast stroma)
18431481(chloroplast)
19525416(leaf (wt and clpr4-1 mutant))
20423899(chloroplast)
19995723(chloroplast reference proteome)

Comparative Proteomics Data

Expr*	Source	Tech.	Spot	PepSeq	ChargeState(z)	AreaRatio	Ambiguity	Tissue	Sample from
195_194	ffc1-2 cICAT- exp1 / wt cICAT- exp1	cICAT	7	VPVLFIAGDRDPLCEVK + ICAT_l / VPVLFIAGDRDPLCEVK + ICAT_h	3	0.98		leaf [A. thaliana]	chloroplast stroma